School of Chemistry and Chemical Engineering, Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, Shandong University, Jinan, 250100, People's Republic of China.
Logistics University of PAP, Tianjin, 300162, People's Republic of China.
Mikrochim Acta. 2018 Jun 6;185(7):317. doi: 10.1007/s00604-018-2844-0.
A nanoprobe was developed for the fluorometric determination of the neurotransmitter dopamine (DA). It is based on the synergistic enhancement action of citrate and gold nanoparticles (AuNPs) on the luminescence of Tb(III). AuNPs serve as substrates of surface enhanced fluorescence (SEF). Citrate, in turn, acts as a spacer for the SEF effect, a co-ligand of Tb(III) complex, and a recognizing component for DA. The synergistic action of citrate and AuNPs significantly increases the intrinsic green fluorescence of Tb(III) (best measured at excitation/emission peaks of 300/547 nm). Under the optimum conditions, the fluorescence intensity increases linearly in the 3.0 to 200 nM DA concentration ranging (with an R value of 0.9959), and the limit of detection (at S/N = 3) is 0.84 nM. The nanoprobe shows good selectivity for DA among other interfering neurotransmitters, some amino acids and ions. The method was applied to the detection of DA in human serum samples where it gave recoveries ranging from 100.5 to 102.9%. Graphical abstract Schematic of a Tb(III) composite fluorescent nanoprobe for the sensitive determination of dopamine (DA). Citrate and gold nanoparticles (AuNPs) synergistically enhance the fluorescence of Tb(III)-DA.
一种用于荧光测定神经递质多巴胺 (DA) 的纳米探针被开发出来。它基于柠檬酸和金纳米粒子 (AuNPs) 对铽 (III) 发光的协同增强作用。AuNPs 用作表面增强荧光 (SEF) 的基底。柠檬酸又作为 SEF 效应的间隔物、Tb(III) 配合物的共配体以及 DA 的识别成分。柠檬酸和 AuNPs 的协同作用显著增加了 Tb(III) 的本征绿色荧光（最佳测量激发/发射峰为 300/547nm）。在最佳条件下，荧光强度在 3.0 至 200nM 的 DA 浓度范围内呈线性增加（R 值为 0.9959），检测限（S/N=3）为 0.84nM。该纳米探针在其他干扰神经递质、一些氨基酸和离子中对 DA 表现出良好的选择性。该方法已应用于人血清样品中 DA 的检测，回收率范围为 100.5%至 102.9%。
