Wang Jiafei, Bai Ligai, Wei Zhen, Qin Junxiao, Ma Yamin, Liu Haiyan
Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, 071002, China.
J Sep Sci. 2015 Jun;38(12):2101-8. doi: 10.1002/jssc.201500061. Epub 2015 May 13.
An ionic liquid was incorporated into the porous polymer monoliths to afford stationary phases with enhanced chromatographic performance for small molecules in reversed-phase high-performance liquid chromatography. The effect of the ionic liquid in the polymerization mixture on the performance of the monoliths was studied in detail. While monoliths without ionic liquid exhibited poor resolution and low efficiency, the addition of ionic liquid to the polymerization mixture provides highly increased resolution and high efficiency. The chromatographic performances of the monoliths were demonstrated by the separations of various small molecules including aromatic hydrocarbons, isomers, and homologues using a binary polar mobile phase. The present column efficiency reached 27 000 plates/m, which showed that the ionic liquid monoliths are alternative stationary phases in the separation of small molecules by high-performance liquid chromatography.
将离子液体引入多孔聚合物整体柱中，以获得在反相高效液相色谱中对小分子具有增强色谱性能的固定相。详细研究了聚合混合物中离子液体对整体柱性能的影响。不含离子液体的整体柱表现出分辨率差和效率低的问题，而向聚合混合物中添加离子液体则可显著提高分辨率和效率。使用二元极性流动相分离包括芳烃、异构体和同系物在内的各种小分子，证明了整体柱的色谱性能。目前的柱效达到27000塔板/米，这表明离子液体整体柱是高效液相色谱分离小分子的替代固定相。
