Tianjin Key Laboratory On Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
Mikrochim Acta. 2022 Feb 7;189(3):85. doi: 10.1007/s00604-022-05192-x.
For the first time a hybrid molecularly imprinted polymer (MIP) doped with 3-(trimethoxysilyl) propyl methacrylate (γ-MPS)-modified mesoporous molecular sieve SBA-15 for target peptide recognition has been developed. Zinc acrylate and methacrylic acid were used as binary functional monomers, and ethylene dimethacrylate was used as cross-linking agent to prepare an imprinted monolith against Val-Tyr-Ala-Leu-Lys(glutarylation) (VYALK). The morphology of the polymers was characterized by scanning electron microscopy, FT-IR spectroscopy, energy dispersive spectroscopy, and H NMR. The SBA-15-MPS MIP showed high recovery of 87.1% and the IF of 12.9 for the enrichment of the template peptide. When the template peptide concentration ranged from 5 to 90 μg mL, the correlation coefficients (R) for the calibration function obtained was better 0.999. The limit of detection (LOD, 0.30 μg mL) and limit of quantification (LOQ, 1.0 μg mL) were achieved for signal-to-noise ratios of 3:1 and 10:1, respectively. When other kinds of synthetic peptides were used as analogs, the selectivity of the SBA-15-MPS MIP was much better than the SBA-15-MPS NIP (without template peptides) with relative selectivity coefficients of 52.8-265. In contrast, little quinolones and biogenic amines are adsorbed with the SBA-15-MPS MIP. The SBA-15-MPS MIP could enrich VYALK from spiked histone digestion with the average recovery of 87.8% and the relative standard deviation (RSD) of 0.99%. As a conclusion, doping of SBA-15 is an effective approach to the improvement of performance of molecularly imprinted monolith.
首次研制出了一种在介孔分子筛 SBA-15 中掺杂 3-(三甲氧基硅基)丙基甲基丙烯酸酯（γ-MPS）的杂化分子印迹聚合物（MIP），用于目标肽识别。以丙烯腈和甲基丙烯酸为二元功能单体，以二乙烯基苯为交联剂，制备了印迹整体柱，用于印迹 Val-Tyr-Ala-Leu-Lys（glutarylation）（VYALK）。通过扫描电子显微镜、傅里叶变换红外光谱、能谱和 H NMR 对聚合物的形态进行了表征。SBA-15-MPS MIP 对模板肽的回收率高达 87.1%，内标因子为 12.9。当模板肽浓度在 5 至 90μg·mL-1 范围内时，校准函数的相关系数（R）较好，为 0.999。当信噪比为 3:1 和 10:1 时，检测限（LOD，0.30μg·mL-1）和定量限（LOQ，1.0μg·mL-1）分别达到。当使用其他种类的合成肽作为类似物时，SBA-15-MPS MIP 的选择性远优于不含模板肽的 SBA-15-MPS NIP（无模板肽），相对选择性系数为 52.8-265。相比之下，SBA-15-MPS MIP 对喹诺酮类和生物胺的吸附量较小。SBA-15-MPS MIP 可以从组蛋白消化物中提取 VYALK，平均回收率为 87.8%，相对标准偏差（RSD）为 0.99%。总之，掺杂 SBA-15 是提高分子印迹整体柱性能的有效方法。
