牛和人类样本中牛结核病的分子诊断：对人畜共患病的影响。
Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis.
作者信息
Rahman Md Masudur, Noor Monira, Islam Kazi Mehetazul, Uddin Md Bashir, Hossain Ferdaus Mohd Altaf, Zinnah Mohammad Ali, Al Mamun Mohammad, Islam Mohammad Rafiqul, Eo Seong Kug, Ashour Hossam M
机构信息
Faculty of Veterinary & Animal Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
出版信息
Future Microbiol. 2015;10(4):527-35. doi: 10.2217/fmb.14.139.
AIM
To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors.
MATERIALS & METHODS: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures.
RESULTS
PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors.
CONCLUSION
Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.
目的
开发用于牛结核病的新兴诊断技术，并确定其潜在风险因素。
材料与方法
从牛乳和人痰液样本中分离细菌基因组DNA，并使用特异性引物对进行PCR。PCR结果通过细菌培养进行验证。
结果
牛分枝杆菌目标DNA片段的PCR扩增在12.33%（37/300）的牛样本中成功。有趣的是，6.67%（6/90）的痰液样本中也扩增出了500bp的DNA片段，表明存在人畜共患病传播的可能性。家庭饲养家畜、饮用生牛奶和吸烟被确定为潜在风险因素。
结论
该研究结果可能为实现“同一健康”倡议的牛结核病根除运动增添价值。
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