比较C18-羧丙基甜菜碱和玻璃珠DNA提取方法用于检测牛乳样品中的牛分枝杆菌及通过聚合酶链式反应（PCR）对样品进行分析。
Comparison of C18-carboxypropylbetaine and glass bead DNA extraction methods for detection of Mycobacterium bovis in bovine milk samples and analysis of samples by PCR.
作者信息
Cornejo B J, Sahagún-Ruiz A, Suárez-Güemes F, Thornton C G, Ficht T A, Adams L G
机构信息
Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
出版信息
Appl Environ Microbiol. 1998 Aug;64(8):3099-101. doi: 10.1128/AEM.64.8.3099-3101.1998.
Abstract
The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.
摘要
这项前瞻性研究的目的是比较两种不同的牛奶制备方法，通过聚合酶链反应（PCR）检测牛分枝杆菌的存在。将基于C18-羧丙基甜菜碱（CB-18）的样品处理方法的检测结果与用玻璃珠从牛奶中提取DNA的结果进行比较。对17头皮肤试验呈阳性的牛的样本进行了分析。经过CB-18处理和玻璃珠提取后，基于IS6110的PCR的灵敏度分别为94.1%和58.8%（P<0.025）。由于CB-18处理将允许熟练使用PCR诊断和监测牛结核病，它将有助于更有效地检测和控制结核病。
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