Hill L E, Yount D J, Garriott M L, Tamura R N, Probst G S
Toxicology Division, Lilly Research Laboratories, Eli Lilly and Company, Greenfield, IN 46140.
Mutat Res. 1989 Dec;224(4):447-51. doi: 10.1016/0165-1218(89)90069-4.
A procedure was developed for the quantification of the autoradiographic assay for unscheduled DNA synthesis. Relative to commonly used practices for grain counting, this procedure provides a more accurate net nuclear grain count by eliminating the subjectivity currently associated with selection of the areas to be counted for the cytoplasmic background count. Briefly, the object area and aperture area modes of an ARTEK 880 colony counter are used to collect values for the total number of silver grains over a particular cell (nuclear and cytoplasmic counts), as well as for the nuclear and cytoplasmic areas. These values are then employed in a short algorithm to determine the net nuclear grain count. This new method provides greater sensitivity for defining weak UDS responses and the data collected readily lends itself to statistical analysis.
已开发出一种用于非预定DNA合成放射自显影测定定量的方法。相对于常用的颗粒计数方法，该方法通过消除目前在选择用于细胞质背景计数的计数区域时存在的主观性，提供了更准确的净核颗粒计数。简而言之，使用ARTEK 880菌落计数器的目标面积和孔径面积模式来收集特定细胞上银颗粒总数（核和细胞质计数）以及核和细胞质面积的值。然后将这些值用于一个简短的算法中以确定净核颗粒计数。这种新方法在定义微弱的非预定DNA合成反应方面具有更高的灵敏度，并且所收集的数据易于进行统计分析。
