Nishijima Tomohiko, Takida Yoshiki, Saito Yasuo, Ikeda Takayuki, Iwai Kunihisa
R&D Division,Glico Dairy Products Company, Limited, 2-14-1 Musashino,Akishima-shi, Tokyo196-0021,Japan.
Faculty of Human Life Sciences, Fuji Women's University,4-5 Hanakawa Minami, Ishikari-shi,Hokkaido061-3204,Japan.
Br J Nutr. 2015 May 28;113(10):1531-8. doi: 10.1017/S0007114515000537. Epub 2015 Apr 13.
Chronic ingestion of apple pectin has been shown to increase the absorption of quercetin in rats. The present study was designed to elucidate whether the simultaneous ingestion of quercetin with apple pectin could enhance the absorption of quercetin in humans, and the effects of dose dependency and degree of pectin methylation on quercetin absorption were also investigated. Healthy volunteers (n 19) received 200 ml of 0.5 mg/ml of quercetin drinks with or without 10 mg/ml of pectin each in a randomised cross-over design study with over 1-week intervals; urine samples from all the subjects were collected within 24 h after ingestion of the test drinks, and urinary deconjugated quercetin and its metabolites were determined using HPLC. The sum of urinary quercetin and its metabolites excreted was increased by 2.5-fold by the simultaneous ingestion of pectin. The metabolism of methylated quercetin (isorhamnetin and tamarixetin) was not affected by pectin ingestion. In six volunteers, who received quercetin drinks containing 0, 3 and 10 mg/ml of pectin, the sum of urinary quercetin and its metabolites excreted also increased in a pectin dose-dependent manner. Furthermore, the simultaneous ingestion of quercetin with low-methoxy and high-methoxy pectin, respectively, increased the sum of urinary excretion of quercetin and its metabolites by 1.69-fold and significantly by 2.13-fold compared with the ingestion of quercetin without pectin. These results elucidated that apple pectin immediately enhanced quercetin absorption in human subjects, and that its enhancing effect was dependent on the dose and degree of pectin methylation. The results also suggested that the viscosity of pectin may play a role in the enhancement of quercetin absorption.
长期摄入苹果果胶已被证明可增加大鼠体内槲皮素的吸收。本研究旨在阐明同时摄入槲皮素和苹果果胶是否能增强人体对槲皮素的吸收，同时还研究了剂量依赖性和果胶甲基化程度对槲皮素吸收的影响。19名健康志愿者参与了一项随机交叉设计研究，研究间隔超过1周，他们分别饮用含有或不含10mg/ml果胶的200ml、0.5mg/ml的槲皮素饮料；在摄入测试饮料后的24小时内收集所有受试者的尿液样本，并使用高效液相色谱法测定尿液中去结合的槲皮素及其代谢产物。同时摄入果胶使排出的尿液中槲皮素及其代谢产物的总量增加了2.5倍。甲基化槲皮素（异鼠李素和柽柳素）的代谢不受果胶摄入的影响。在6名分别饮用含有0、3和10mg/ml果胶的槲皮素饮料的志愿者中，排出的尿液中槲皮素及其代谢产物的总量也呈现出果胶剂量依赖性增加。此外，与不摄入果胶而只摄入槲皮素相比，同时分别摄入低甲氧基果胶和高甲氧基果胶时，尿液中槲皮素及其代谢产物的排泄总量分别增加了1.69倍和显著的2.13倍。这些结果表明，苹果果胶能立即增强人体对槲皮素的吸收，且其增强作用取决于果胶的剂量和甲基化程度。结果还表明，果胶的粘度可能在增强槲皮素吸收方面发挥作用。
