Pham Ngan Thi Kim, Trinh Quang Duy, Khamrin Pattara, Ukarapol Nuthapong, Kongsricharoern Tipachan, Yamazaki Wataru, Komine-Aizawa Shihoko, Okitsu Shoko, Maneekarn Niwat, Hayakawa Satoshi, Ushijima Hiroshi
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.
Jpn J Infect Dis. 2015;68(5):432-3. doi: 10.7883/yoken.JJID.2014.450. Epub 2015 Apr 10.
Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hipO gene (in C. jejuni) or the ask gene (in C. coli). The results suggest that the prevalence of Campylobacter infection among Thai children with diarrhea is 8.6%, and C. jejuni is the most prevalent species.
弯曲杆菌属是细菌性腹泻的常见病因，空肠弯曲杆菌和大肠弯曲杆菌是已知的人类主要致病原。最近的研究表明，环介导等温扩增技术（LAMP）是一种用于快速检测临床样本中空肠弯曲杆菌和大肠弯曲杆菌的高效实用工具。我们使用LAMP对151份粪便样本进行弯曲杆菌筛查；这些样本于2012年从患有腹泻的泰国儿童中收集。PCR方法在检测到的弯曲杆菌菌株中区分空肠弯曲杆菌和大肠弯曲杆菌；对这些菌种进行hipO基因（空肠弯曲杆菌中）或ask基因（大肠弯曲杆菌中）测序。结果表明，患有腹泻的泰国儿童中弯曲杆菌感染率为8.6%，空肠弯曲杆菌是最常见的菌种。
