用于检测犬粪便样本中DNA（粪便环介导等温扩增法）的新型环介导等温扩增检测方法的开发。
Development of a New LAMP Assay for the Detection of DNA (Copro-LAMPAc) in Dog Fecal Samples.
作者信息
Avila Héctor Gabriel, Risso Marikena Guadalupe, Cabrera Marta, Ruybal Paula, Repetto Silvia Analía, Butti Marcos Javier, Trangoni Marcos David, Santillán Graciela, Pérez Verónica Mirtha, Periago María Victoria
机构信息
Laboratorio Provincial de Zoonosis de San Juan, Facultad de Ciencias Veterinarias, Facultad de Ciencias Químicas y Tecnológicas, Universidad Católica de Cuyo, San Juan, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
出版信息
Front Vet Sci. 2021 Nov 12;8:770508. doi: 10.3389/fvets.2021.770508. eCollection 2021.
is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the -1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of for epidemiological studies in endemic areas.
是一种人畜共患的线虫，能够感染动物和人类。在终末宿主中进行诊断和环境检测是预防其传播并实现控制的关键。在此，开发了一种用于检测（粪便LAMP检测蛔虫）DNA的新粪便学环介导等温扩增方法。使用低成本方法进行DNA提取，并使用-1基因的片段进行引物设计。用来自成年蠕虫的基因组DNA系列稀释液评估的分析灵敏度为100 fg。使用来自宿主和其他病原体的基因组DNA获得了100%的特异性。使用环境犬粪便样本对粪便LAMP检测蛔虫进行了评估。在流行病学研究中与金标准光学显微镜相比，它被证明更敏感。这种新的环介导等温扩增检测方法可以为流行地区流行病学研究中蛔虫的筛查和鉴定提供一种替代方案。
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