Suwancharoen Duangjai, Limlertvatee Supaluck, Chetiyawan Philaiphon, Tongpan Phichet, Sangkaew Nongluck, Sawaddee Yaowarat, Inthakan Kanya, Wiratsudakul Anuwat
National Institute of Animal Health, Department of Livestock Development, 50/2 Kasetklang, Ladyao, Chatuchak, Bangkok 10900, Thailand.
J Vet Med Sci. 2016 Oct 1;78(9):1495-1500. doi: 10.1292/jvms.15-0493. Epub 2016 Jun 13.
Leptospirosis is a worldwide distributed zoonosis which has long been endemic in Thailand. Cattle and buffaloes are important livestock species that live in close contact with humans, especially in rural areas. These animals may, therefore, act as long-term carriers of leptospirosis for humans and other livestock species. The present study employed loop-mediated isothermal amplification (LAMP) method to detect pathogenic leptospiral 16S rDNA in the urine of cattle and buffaloes for assessing associations between uroprevalence and species, sex, age and spatial distribution. A total of 3,657 urine samples were collected for laboratory diagnosis, and 312 of which turned positive to the test (true prevalence 5.90%; 95% CI 4.98-6.91). The highest true uroprevalence was found in lower northern region at 19.80% (95% CI 15.83-24.32) followed by upper and lower northeastern regions at 15.22% and 6.25%, respectively. However, the highest true uroprevalence in beef cattle, the majority of cattle in Thailand, was recorded in northeastern region which is the endemic area of human leptospirosis. The uroprevalence was not statistically different among species and types of examined animals. Male animals were over twice more likely to be infected compared to females. Excluding animals younger than one year of age due to small sample size, the uroprevalence upraised with increasing age. A collaborative investigation between veterinary and public health sectors is required to holistically explore the link between leptospirosis in humans and livestock, especially in high prevalent areas.
钩端螺旋体病是一种分布于全球的人畜共患病，在泰国长期流行。牛和水牛是与人类密切接触的重要家畜品种，尤其是在农村地区。因此，这些动物可能成为人类和其他家畜品种钩端螺旋体病的长期携带者。本研究采用环介导等温扩增（LAMP）方法检测牛和水牛尿液中致病性钩端螺旋体16S rDNA，以评估尿患病率与物种、性别、年龄和空间分布之间的关联。共收集3657份尿液样本进行实验室诊断，其中312份检测呈阳性（实际患病率5.90%；95%可信区间4.98 - 6.91）。北部较低地区的实际尿患病率最高，为19.80%（95%可信区间15.83 - 24.32），其次是东北部上、下地区，分别为15.22%和6.25%。然而，泰国大多数牛种肉牛的实际尿患病率最高的地区是人类钩端螺旋体病的流行区东北部。所检查动物的物种和类型之间的尿患病率在统计学上没有差异。雄性动物感染的可能性是雌性动物的两倍多。由于样本量小，排除一岁以下的动物后，尿患病率随年龄增长而升高。兽医和公共卫生部门需要进行合作调查，以全面探索人类和家畜钩端螺旋体病之间的联系，特别是在高流行地区。
