Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041 China.
University of Chinese Academy of Sciences, Beijing, 100049 China.
Biotechnol Biofuels. 2015 Apr 2;8:57. doi: 10.1186/s13068-015-0246-7. eCollection 2015.
Duckweed is a novel aquatic bioenergy crop that is found ubiquitously throughout the world. Uniconazole plays an important role in improving crop production through the regulation of endogenous hormone levels. We found that a high quantity and quality of duckweed growth can be achieved by uniconazole application, although the mechanisms are unknown.
The fronds of Landoltia punctata were sprayed evenly with 800 mg/L uniconazole. The dry weight following treatment increased by 10% compared to the controls at 240 h. Endogenous cytokinin (CK) and abscisic acid (ABA) content both increased compared to the control, while the level of gibberellins (GAs) decreased. Additionally, gene expression profiling results showed that the expression of transcripts encoding key enzymes involved in endogenous CK and ABA biosynthesis were up-regulated, while the transcripts of key enzymes for GAs biosynthesis were down-regulated. On the other hand, chlorophyll a and chlorophyll b contents were both increased compared with the control. Moreover, the net photosynthetic rate was elevated to 25.6 μmol CO2/m(2)/s compared with the control value of 22.05 μmol CO2/m(2)/s. Importantly, the expression of some chlorophyll biosynthesis-related transcripts was up-regulated.
Uniconazole treatment altered endogenous hormone levels and enhanced chlorophyll content and net photosynthetic rate in duckweed by regulating key enzymes involved in endogenous hormone and chlorophyll biosynthesis. The alterations of endogenous hormones and the increase of chlorophyll and photosynthetic rate data support the increase of biomass and starch accumulation.
浮萍是一种新型水生生物能源作物，在世界各地广泛存在。烯效唑通过调节内源激素水平在提高作物产量方面发挥着重要作用。我们发现，烯效唑的应用可以实现浮萍生长的高数量和高质量，尽管其机制尚不清楚。
将 Landoltia punctata 的叶片均匀喷洒 800mg/L 的烯效唑。与对照组相比，处理 240 小时后，干重增加了 10%。与对照组相比，内源细胞分裂素（CK）和脱落酸（ABA）含量均增加，而赤霉素（GA）水平降低。此外，基因表达谱结果表明，参与内源 CK 和 ABA 生物合成的关键酶编码转录物的表达上调，而参与 GA 生物合成的关键酶的转录物下调。另一方面，与对照组相比，叶绿素 a 和叶绿素 b 的含量均增加。此外，净光合速率提高到 25.6μmol CO2/m(2)/s，而对照组的值为 22.05μmol CO2/m(2)/s。重要的是，一些与叶绿素生物合成相关的转录物的表达上调。
烯效唑处理通过调节内源激素和叶绿素生物合成关键酶，改变内源激素水平，提高浮萍叶绿素含量和净光合速率。内源激素的改变和叶绿素及光合速率的增加数据支持生物量和淀粉积累的增加。
