人巨细胞病毒UL49基因座可变剪接的鉴定

Identification of the alternative splicing of the UL49 locus of human cytomegalovirus.

作者信息

Yang Guang, Li Wei, Liao Wenzhen, Zhang Xin, Zou Yi, Dai Jianfeng, Li Yueqin, Jing Chunxia, Zhou Tianhong

机构信息

Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China ; Department of Parasitology, Medical School, Jinan University, Guangzhou 510632, China.

Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

出版信息

Biomed Res Int. 2015;2015:280276. doi: 10.1155/2015/280276. Epub 2015 Mar 19.

DOI:10.1155/2015/280276

PMID:25866769

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4383306/

Abstract

The UL49 ORF of human cytomegalovirus (HCMV) is essential for viral replication; conserved among all herpes viruses; however, the function is unclear. Once the UL49 ORF was precisely deleted from the start to stop codon, the mutant did not yield infectious progeny. In this study, we find out many alternatively processed ESTs in UL49 locus in HCMV-infected cells, in which there are two novel transcription termination sites in UL49 locus. Most of these ESTs are rare transcripts that contain directed repeat sequences in the intron splicing regions. There is a typical GU-AG intron splicing site in UL49Y transcripts. The 1847 bp UL49Y cDNA spans an ORF from 335 to 1618 and encodes a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa. All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos. GW314860-GW314900 and GU376796). This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

摘要

人类巨细胞病毒（HCMV）的UL49开放阅读框对于病毒复制至关重要；在所有疱疹病毒中都保守；然而，其功能尚不清楚。一旦从起始密码子到终止密码子精确删除UL49开放阅读框，该突变体就不会产生有感染性的子代。在本研究中，我们在HCMV感染细胞的UL49基因座中发现了许多可变加工的EST，其中UL49基因座中有两个新的转录终止位点。这些EST中的大多数是罕见转录本，在内含子剪接区域含有定向重复序列。UL49Y转录本中有一个典型的GU-AG内含子剪接位点。1847 bp的UL49Y cDNA跨越一个从335到1618的开放阅读框，编码一个推定的427个氨基酸的蛋白质，预测分子量为47.1 kDa。所有新的EST序列和UL49Y cDNA序列已存入GenBank数据库（GenBank登录号：GW314860-GW314900和GU376796）。本研究为揭示UL49基因座可变剪接的重要性提供了非常重要的线索。