STAG2缺失导致正常人膀胱细胞出现非整倍体。

Loss of STAG2 causes aneuploidy in normal human bladder cells.

作者信息

Li X, Zhang T W, Tang J L, Fa P P, Lu J X, Qi F M, Cai Z M, Liu C X, Sun X J

机构信息

Department of Urology, Zhujiang Hospital, Second Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.

Shenzhen Tumor Immuno-Gene Therapy Clinical Application Engineering Laboratory, Biobank of Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong Province, China.

出版信息

Genet Mol Res. 2015 Mar 30;14(1):2638-46. doi: 10.4238/2015.March.30.24.

DOI:10.4238/2015.March.30.24

PMID:25867412

Abstract

The aim of this study was to determine how the function of human stromal antigen 2 (STAG2) plays an important role in proper chromosome separation. STAG2 mRNA in normal bladder cells and bladder tumor cells was evaluated by RT-PCR. The protein levels of STAG2 in normal bladder cells and bladder tumor cells were determined by western blot. A cell proliferation assay was used to measure the growth of tumor cells and STAG2-inhibited normal cells, and STAG2- inhibited normal cells were subjected to karyotype analysis. Both STAG-2 mRNA and protein expression levels were lower in bladder cancer cells compared to the controls. Knockdown of STAG2 caused aneuploidy in normal bladder cells, leading to a decreased expression of the cohesin complex components SMC1, SMC3 and RAD21, but there was no obvious effect of STAG2 knockdown on cell proliferation. Our study indicated that abnormal expression of STAG2 could cause aneuploidy in normal bladder cells.

摘要

本研究的目的是确定人类基质抗原2（STAG2）的功能如何在正确的染色体分离中发挥重要作用。通过逆转录聚合酶链反应（RT-PCR）评估正常膀胱细胞和膀胱肿瘤细胞中STAG2 mRNA的水平。通过蛋白质印迹法测定正常膀胱细胞和膀胱肿瘤细胞中STAG2的蛋白质水平。使用细胞增殖试验来测量肿瘤细胞和STAG2抑制的正常细胞的生长，并对STAG2抑制的正常细胞进行核型分析。与对照组相比，膀胱癌细胞中STAG-2 mRNA和蛋白质表达水平均较低。敲低STAG2会导致正常膀胱细胞出现非整倍体，导致黏连蛋白复合体成分SMC1、SMC3和RAD21的表达降低，但敲低STAG2对细胞增殖没有明显影响。我们的研究表明，STAG2的异常表达可导致正常膀胱细胞出现非整倍体。