狼尾草属不同品种蔗糖磷酸合成酶基因的克隆与序列分析

Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species.

作者信息

Li H C, Lu H B, Yang F Y, Liu S J, Bai C J, Zhang Y W

机构信息

Beijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, China.

Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan, China.

出版信息

Genet Mol Res. 2015 Mar 31;14(1):2799-808. doi: 10.4238/2015.March.31.10.

DOI:10.4238/2015.March.31.10

PMID:25867429

Abstract

Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott). The cloned sequences showed 99.9% identity and 80-88% similarity to the SPS sequences of other plants. The SPS gene of hybrid Pennisetum had one nucleotide and four amino acid polymorphisms compared to the other four germplasms, and cluster analysis was performed to assess genetic diversity in this species. Additional characterization of the SPS gene product can potentially allow Pennisetum to be exploited as a biofuel source.

摘要

蔗糖磷酸合酶（SPS）是高等植物用于合成蔗糖的一种酶。在本研究中，基于玉米（基因库：NM_001112224.1）和甘蔗（基因库：JN584485.1）已知的SPS序列设计了三套引物，并通过RT-PCR从狼尾草属植物（杂交种美洲狼尾草×紫狼尾草、紫狼尾草、紫狼尾草品种红、紫狼尾草品种台湾和紫狼尾草品种莫特）的基因组中鉴定出五个新的SPS基因。克隆的序列与其他植物的SPS序列显示出99.9%的同一性和80 - 88%的相似性。与其他四种种质相比，杂交狼尾草的SPS基因有一个核苷酸和四个氨基酸多态性，并进行了聚类分析以评估该物种的遗传多样性。对SPS基因产物的进一步表征可能使狼尾草被开发为生物燃料来源。