Gutiérrez-Vergara C, Quintero J, Duarte J F, Suescún J P, López-Herrera A
Laboratory of Animal Biotechnology, BIOGEM Research Group, Animal Production Department, Universidad Nacional de Colombia, Medellín, Antioquia, Colombia
Laboratory of Animal Biotechnology, BIOGEM Research Group, Animal Production Department, Universidad Nacional de Colombia, Medellín, Antioquia, Colombia.
Genet Mol Res. 2015 Mar 31;14(1):2890-9. doi: 10.4238/2015.March.31.20.
Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers sold in grocery stores are contaminated with HEV. It is also known that HEV can be inactivated when cooked, as it is labile to high temperatures. The aim of this study was to determine HEV contamination in pig livers sold in Medellín, Antioquia. A total of 150 livers from 5 slaughterhouses and 100 livers in grocery stores from different social strata of the city of Medellin analyzed to detect a segment of the HEV open reading frame-1 using reverse transcription-polymerase chain reaction. The results showed that 41.3% of pig livers from slaughterhouses and 25% of livers from grocery stores tested positive for HEV. Thus, the HEV genome is present in pig livers sold in Antioquia, revealing the presence of this virus in pigs from Colombia and the need subject entrails to proper cooking processes before consumption. Further research is required to determine the role of this virus in public health and pork production in Colombia.
戊型肝炎是一种在世界许多国家的人类中发现的地方性急性肝炎，由戊型肝炎病毒（HEV）引起。在猪身上检测到HEV表明它们可能是携带者，可能通过人畜共患病传播。哥伦比亚猪群中HEV的流行情况尚不清楚。美国的研究发现，杂货店出售的猪肝中有11%被HEV污染。还已知HEV在烹饪时可被灭活，因为它对高温不稳定。本研究的目的是确定在安蒂奥基亚省麦德林市出售的猪肝中的HEV污染情况。使用逆转录-聚合酶链反应对来自5家屠宰场的150份肝脏以及麦德林市不同社会阶层杂货店的100份肝脏进行分析，以检测HEV开放阅读框-1的一个片段。结果显示，屠宰场的猪肝中有41.3%以及杂货店的猪肝中有25%的HEV检测呈阳性。因此，安蒂奥基亚省出售的猪肝中存在HEV基因组，这表明哥伦比亚的猪身上存在这种病毒，也表明在食用前需要对猪内脏进行适当的烹饪处理。需要进一步研究以确定这种病毒在哥伦比亚公共卫生和猪肉生产中的作用。
