Pow D V, Morris J F
Department of Human Anatomy, University of Oxford, U.K.
Neuroscience. 1989;32(2):435-9. doi: 10.1016/0306-4522(89)90091-2.
Exocytosis of neurosecretory granules from dendrites of magnocellular neurons can be visualized electron microscopically after incubation of hypothalamic brain slices in media containing 1.2 mM tannic acid, which stabilizes extracellular peptidergic granule cores, and permits their immunocytochemical identification. Morphometric analysis of stimulated slices demonstrates that exocytosis of neurosecretory granules from the dendrites of magnocellular neurons can account for the vasopressin and oxytocin known to be released into the hypothalamus. Exocytosis from cell bodies of magnocellular neurons was not observed in stimulated slices from normal rats but, when granules had been caused to accumulate in the neuronal somata by prior administration of colchicine, exocytosis of granules from the somata was unambiguously identified. These data demonstrate exocytosis from dendrites and cell bodies of a mammalian peptidergic neuron, and show that all parts of their plasmalemma are competent for exocytosis of granules.
将下丘脑脑片置于含有1.2 mM鞣酸的培养基中孵育后，可通过电子显微镜观察到来自大细胞神经元树突的神经分泌颗粒的胞吐作用。鞣酸可稳定细胞外肽能颗粒核心，并允许对其进行免疫细胞化学鉴定。对受刺激脑片的形态计量分析表明，大细胞神经元树突的神经分泌颗粒胞吐作用可解释已知释放到下丘脑的血管加压素和催产素的来源。在正常大鼠的受刺激脑片中未观察到来自大细胞神经元细胞体的胞吐作用，但是，当通过预先给予秋水仙碱使颗粒在神经元胞体中积累时，可明确鉴定出胞体中颗粒的胞吐作用。这些数据证明了哺乳动物肽能神经元树突和细胞体的胞吐作用，并表明其质膜的所有部分都具有颗粒胞吐作用的能力。
