Armstrong W E, Schöler J, McNeill T H
Neuroscience. 1982 Mar;7(3):679-94. doi: 10.1016/0306-4522(82)90074-4.
Processes of magnocellular neurosecretory cells in the rat supraoptic nucleus which project along the pial surface in the ventral glial lamina were investigated using immunocytochemistry, Golgi stains and electron microscopy. Immunocytochemical studies revealed that although both oxytocin- and vasopressin-containing processes were evident in the ventral glial lamina, vasopressin-containing processes predominated. Ventral processes were thicker and of a different morphology than dorsal axon-like processes which joined the hypothalamo-neurohypophysial tract and exhibited large varicosities along their length or at their apparent termination. Golgi stains revealed that classically defined dendrites of supraoptic neurons projected primarily ventrally and often invaded the ventral glial lamina. No axons were traced to the lamina. Ultrastructurally, processes within the ventral glial lamina characterized as dendrites could be stained immunocytochemically for neurophysin and were post-synaptic to a variety of presynaptic elements. The results suggest that many dendrites from magnocellular neurosecretory cells in the supraoptic nucleus project to the ventral glial lamina and form a restricted, receptive plexus. The previously demonstrated coexistence of catecholamine-containing varicosities and other axon types with these processes in the lamina indicates an important role for supraoptic dendrites in integrating a wide variety of information relevant to neurohypophysial hormone release.
利用免疫细胞化学、高尔基染色法和电子显微镜技术，对大鼠视上核中沿腹侧神经胶质板软膜表面投射的大细胞神经分泌细胞的过程进行了研究。免疫细胞化学研究表明，虽然在腹侧神经胶质板中含催产素和含血管加压素的过程都很明显，但含血管加压素的过程占主导。腹侧过程比背侧轴突样过程更粗且形态不同，背侧轴突样过程与下丘脑 - 神经垂体束相连，并在其长度或明显的末端表现出大的膨体。高尔基染色显示，视上神经元经典定义的树突主要向腹侧投射，并经常侵入腹侧神经胶质板。没有轴突追踪到该板层。超微结构上，腹侧神经胶质板内被表征为树突的过程可以用免疫细胞化学方法对神经垂体素进行染色，并且是多种突触前成分的突触后成分。结果表明，视上核中许多大细胞神经分泌细胞的树突投射到腹侧神经胶质板并形成一个受限的感受性神经丛。先前已证明在该板层中含儿茶酚胺的膨体和其他轴突类型与这些过程共存，这表明视上核树突在整合与神经垂体激素释放相关的各种信息方面具有重要作用。
