多酚类化合物作为细胞毒性和黄曲霉毒素 A 诱导的 DNA 损伤的可能生物保护剂。
Polyphenols as possible bioprotectors against cytotoxicity and DNA damage induced by ochratoxin A.
机构信息
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, Ciudad Autónoma de Buenos Aires, CP C1033AAJ, Argentina.
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, Ciudad Autónoma de Buenos Aires, CP C1033AAJ, Argentina.
出版信息
Environ Toxicol Pharmacol. 2015 May;39(3):1008-18. doi: 10.1016/j.etap.2015.03.013. Epub 2015 Mar 28.
The present study aimed to investigate the protective effects of luteolin (L), chlorogenic acid (ChlA) and caffeic acid (CafA) against cyto-genotoxic effects caused by OTA. Vero cells and rat lymphocytes were used and viability was measured by neutral red uptake, MTT and trypan blue dye exclusion method. L (50 and 100μg/mL), ChlA (100 and 200μg/mL) and CafA (10-50μg/mL) reduced the damage induced by OTA (10μg/mL) on both cells type shown a good protective effect. The comet and micronucleus tests in Balb/c mice were performed. ChlA (10mg/kg bw) reduced OTA (0.85mg/kg bw)-induced DNA damage on blood and bone marrow cells, CafA (10mg/kg bw) showed protective effect only in blood cells and luteolin (2.5mg/kg bw) failed to protect DNA integrity on cells. In conclusion, polyphenols tested reduced the toxicity caused by OTA on different target cells with good protective effect, being ChlA the compound that showed the best effects.
本研究旨在探讨木犀草素（L）、绿原酸（ChlA）和咖啡酸（CafA）对OTA 引起的细胞遗传毒性的保护作用。使用vero 细胞和大鼠淋巴细胞，通过中性红摄取、MTT 和台盼蓝排斥试验测定细胞活力。L（50 和 100μg/ml）、ChlA（100 和 200μg/ml）和 CafA（10-50μg/ml）降低了 OTA（10μg/ml）对两种细胞类型的损伤，表现出良好的保护作用。在 Balb/c 小鼠中进行了彗星和微核试验。ChlA（10mg/kg bw）降低了 OTA（0.85mg/kg bw）引起的血液和骨髓细胞的 DNA 损伤，CafA（10mg/kg bw）仅在血液细胞中表现出保护作用，而木犀草素（2.5mg/kg bw）未能保护细胞的 DNA 完整性。总之，测试的多酚对不同靶细胞的 OTA 毒性具有良好的保护作用，其中 ChlA 表现出最好的效果。
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