Karaman Ali, Aydın Hatip, Geçkinli Bilge, Çetinkaya Arda, Karaman Selin
Department of Medical Genetics, Istanbul Zeynep Kamil Women and Children Training and Research Hospital, Istanbul, Turkey.
Department of Medical Genetics, Istanbul Zeynep Kamil Women and Children Training and Research Hospital, Istanbul, Turkey.
Mutat Res Genet Toxicol Environ Mutagen. 2015 Apr;782:30-5. doi: 10.1016/j.mrgentox.2015.03.009. Epub 2015 Mar 13.
We assessed DNA damage in patients with metabolic syndrome (MetS) by performing comet and micronucleus (MN) assays on peripheral blood lymphocyte cultures from study participants. 52 MetS patients and 35 age-matched healthy controls were evaluated for abdominal obesity, body-mass index (BMI), blood pressure, serum triglycerides, HbA1c, HDL-C, and fasting blood glucose levels. In addition, malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined. Serum levels of triglycerides, HbA1c, fasting blood glucose and waist circumference, systolic blood pressure, diastolic blood pressure, and BMI of the subjects in the MetS group were significantly higher than those of the control group (for each, p<0.001). However, the mean level of HDL-C in the MetS group was lower than in the control group (p<0.001). In the study, the length of comet tails was significantly higher in the MetS patients (10.23±1.98, range 5.72-15.08) than in the controls (3.12±1.73, range 0.6-7.1) (p<0.001). MN frequency was also significantly increased in MetS patients (3.68±1.27 per 1000 cells) compared to that of the control group (1.81±0.84 per 1000 cells) (p<0.001). Micronucleated cell frequency and comet-tail length in subjects showed positive correlations with waist circumference, BMI, and plasma triglyceride levels (p<0.01) and negative correlations with HDL-C levels (p<0.01). Among the oxidative stress factors, MDA levels were significantly higher in MetS patients than in the controls. However, SOD and GSH-Px enzyme activities were significantly lower in the MetS group than in the controls. These findings suggest that patients with MetS have increased DNA damage and oxidative stress.
我们通过对研究参与者外周血淋巴细胞培养物进行彗星试验和微核（MN）试验，评估了代谢综合征（MetS）患者的DNA损伤情况。对52名MetS患者和35名年龄匹配的健康对照者进行了腹部肥胖、体重指数（BMI）、血压、血清甘油三酯、糖化血红蛋白（HbA1c）、高密度脂蛋白胆固醇（HDL-C）和空腹血糖水平的评估。此外，还测定了丙二醛（MDA）水平以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。MetS组受试者的血清甘油三酯、HbA1c、空腹血糖水平以及腰围、收缩压、舒张压和BMI均显著高于对照组（每项p<0.001）。然而，MetS组的HDL-C平均水平低于对照组（p<0.001）。在该研究中，MetS患者的彗星尾长度（10.23±1.98，范围5.72 - 15.08）显著高于对照组（3.12±1.73，范围0.6 - 7.1）（p<0.001）。与对照组（每1000个细胞中1.81±0.84）相比，MetS患者的微核频率也显著增加（每1000个细胞中3.68±1.27）（p<0.001）。受试者的微核细胞频率和彗星尾长度与腰围、BMI和血浆甘油三酯水平呈正相关（p<0.01），与HDL-C水平呈负相关（p<0.01）。在氧化应激因素中，MetS患者的MDA水平显著高于对照组。然而，MetS组的SOD和GSH-Px酶活性显著低于对照组。这些发现表明，MetS患者的DNA损伤和氧化应激增加。
