Wang Yan-Hong, Li Tie-Hua, Wu Ben-Quan, Liu Hui, Shi Yun-Feng, Feng Ding-Yun
Department of Medical Intensive Care Unit, The Third Affiliated Hospital of Sun Yat-Sen University , Guangzhou, Guangdong Province , PR China.
Pharm Biol. 2015;53(9):1367-71. doi: 10.3109/13880209.2014.982300. Epub 2015 Apr 14.
The fruit of Xanthium strumarium L. (Asteraceae) has been used for the treatment of various inflammatory diseases.
This study investigates the protective effect of caffeoylxanthiazonoside (CYXD) isolated from fruits of X. strumarium on sepsis mice in vitro and in vivo.
Cecal ligation and puncture (CLP) operation was used to establish the sepsis mice model, and sham mice were also performed. CYXD was administered by intraperitoneal injection (10, 20, and 40 mg/kg/d), then the survival rate was measured in 96 h. Additionally, sepsis mice were induced by injection LPS (2 mg/kg); CYXD was administered by intraperitoneal injection (10, 20, and 40 mg/kg/d), then mice were sacrificed, and serum levels of TNF-α and IL-6 were determined by ELISA assay. Furthermore, the ability of CYXD to neutralize LPS was measured by using the LAL test, and expressions of TNF-α, IL-6 were determined by using real-time fluorogenic PCR.
Results indicated that CYXD significantly elevated survival rates of sepsis mice induced by CLP (p < 0.05) with survival rates of 35%, 45%, and 65%. Furthermore, the LPS level was decreased obviously by CYXD (1, 2, and 4 mg/L) (p < 0.05). Additionally, CYXD (10, 20, and 40 mg/kg) can not only significantly decrease TNF-α and IL-6 levels induced by LPS in mice's serum (p < 0.05), but also inhibit mRNA expressions of TNF-α and IL-6 induced by LPS in RAW 264.7 cells at doses of 20, 40, and 80 μg/mL (p < 0.05).
Our study demonstrated that CYXD has significant protective effects on sepsis mice.
苍耳（菊科）果实已被用于治疗各种炎症性疾病。
本研究调查从苍耳果实中分离出的咖啡酰苍耳酮苷（CYXD）在体外和体内对脓毒症小鼠的保护作用。
采用盲肠结扎穿孔（CLP）手术建立脓毒症小鼠模型，同时设假手术小鼠。通过腹腔注射给予CYXD（10、20和40mg/kg/d），然后在96小时内测量生存率。此外，通过注射脂多糖（LPS，2mg/kg）诱导脓毒症小鼠；通过腹腔注射给予CYXD（10、20和40mg/kg/d），然后处死小鼠，采用酶联免疫吸附测定法（ELISA）测定血清中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平。此外，采用鲎试剂法测定CYXD中和LPS的能力，采用实时荧光定量PCR法测定TNF-α、IL-6的表达。
结果表明，CYXD显著提高了CLP诱导的脓毒症小鼠的生存率（p<0.05），生存率分别为35%、45%和65%。此外，CYXD（1、2和4mg/L）明显降低了LPS水平（p<0.05）。此外，CYXD（10、20和40mg/kg）不仅能显著降低LPS诱导的小鼠血清中TNF-α和IL-6水平（p<0.05），还能在20、40和80μg/mL剂量下抑制LPS诱导的RAW 264.7细胞中TNF-α和IL-6的mRNA表达（p<0.05）。
我们的研究表明，CYXD对脓毒症小鼠具有显著的保护作用。
