Choi Ki-Choon, Son Young-Ok, Hwang Jung-Min, Kim Beom-Tae, Chae Minseon, Lee Jeong-Chae
a Grassland and Forages Research Center , National Institute of Animal Science , Cheonan , South Korea.
b Cell Dynamics Research Center and School of Life Sciences , Gwangju Institute of Science and Technology , Gwangju , South Korea.
Pharm Biol. 2017 Dec;55(1):611-619. doi: 10.1080/13880209.2016.1266673.
Interest has recently renewed in using Lolium multiflorum Lam. (Poaceae) (called Italian ryegrass; IRG) silage as an antioxidant and anti-inflammatory diet.
This study investigated the antioxidant, anti-inflammatory and anti-septic potential of IRG silage and identified the primary components in IRG active fractions.
Total 16 fractions were separated from the chloroform-soluble extract of IRG aerial part using Sephadex LH-20 column before HPLC analysis. Antioxidant and anti-inflammatory activities of the fractions at doses of 0-100 μg/mL were investigated using various cell-free and cell-mediated assay systems. To explore anti-septic effect of IRG fractions, female ICR and BALB/c mice orally received 40 mg/kg of phenolic acid and flavonoid-rich active fractions F and F every other day for 10 days, respectively, followed by LPS challenge.
The active fractions showed greater antioxidant and anti-inflammatory potential compared with other fractions. IC values of F and F to reduce LPS-stimulated NO and TNF-α production were around 15 and 30 μg/mL, respectively. Comparison of retention times with authentic compounds through HPLC analysis revealed the presence of caffeic acid, ferulic acid, myricetin and kaempferol in the fractions as primary components. These fractions inhibited LPS-stimulated MAPK and NF-κB activation. Supplementation with F or F improved the survival rates of mice to 70 and 60%, respectively, in LPS-injected mice and reduced near completely serum TNF-α and IL-6 levels.
This study highlights antioxidant, anti-inflammatory and anti-septic activities of IRG active fractions, eventually suggesting their usefulness in preventing oxidative damage and inflammatory disorders.
最近人们对使用多花黑麦草（禾本科）（称为意大利黑麦草；IRG）青贮饲料作为抗氧化和抗炎饮食的兴趣再度兴起。
本研究调查了IRG青贮饲料的抗氧化、抗炎和抗菌潜力，并确定了IRG活性组分中的主要成分。
在进行高效液相色谱（HPLC）分析之前，使用葡聚糖凝胶LH - 20柱从IRG地上部分的氯仿可溶提取物中分离出总共16个组分。使用各种无细胞和细胞介导的检测系统研究了剂量为0 - 100μg/mL的这些组分的抗氧化和抗炎活性。为了探究IRG组分的抗菌作用，雌性ICR和BALB/c小鼠分别每隔一天口服40mg/kg富含酚酸和黄酮类的活性组分F和F，持续10天，随后进行脂多糖（LPS）攻击。
与其他组分相比，活性组分显示出更大的抗氧化和抗炎潜力。F和F降低LPS刺激的一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）产生的半数抑制浓度（IC）值分别约为15和30μg/mL。通过HPLC分析将保留时间与标准化合物进行比较，结果显示这些组分中主要成分有咖啡酸、阿魏酸、杨梅素和山奈酚。这些组分抑制了LPS刺激的丝裂原活化蛋白激酶（MAPK）和核因子κB（NF-κB）的激活。补充F或F分别将LPS注射小鼠的存活率提高到了70%和60%，并几乎完全降低了血清TNF-α和白细胞介素-6（IL-6）水平。
本研究突出了IRG活性组分的抗氧化、抗炎和抗菌活性，最终表明它们在预防氧化损伤和炎症性疾病方面的有用性。
