Jeong Hae Jin, Lee Hyo Jin, Hong Seongjin, Khim Jong Seong, Shim Won Joon, Kim Gi Beum
Department of Marine Environmental Engineering, Gyeongsang National University, Tongyeong, Republic of Korea.
School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Mar Pollut Bull. 2015 Jun 15;95(1):452-7. doi: 10.1016/j.marpolbul.2015.03.031. Epub 2015 Apr 11.
We examined the degree of DNA damage caused by three fractions (F1, aliphatic hydrocarbons; F2, aromatic hydrocarbons; and F3, polar compounds) of the organic extract of sediments taken from Taean, Korea, following the Hebei Spirit oil spill. DNA damage was measured using the comet assay with blood cells of the striped beakfish (Oplegnathus fasciatus). DNA damage was also examined for fractions of crude oil (Iranian Heavy Crude Oil, IHC), weathered oil and six subfractions (F2.1-F2.6). The greatest DNA damage was found from the Sinduri dune region and DNA damage decreased to 40% weathered oil in F2 fraction compared with crude oil. The DNA damage of the sum of fractions was found higher than the organic extracts of sediments, suggesting antagonistic interactions between the genotoxic compounds. This study confirmed the persistence of potential genotoxicity in sediments of the severely affected regions as long as 5 years after the oil spill.
我们检测了韩国泰安在河北精神号油轮漏油事件后采集的沉积物有机提取物的三个组分（F1，脂肪烃；F2，芳香烃；F3，极性化合物）所造成的DNA损伤程度。使用彗星试验，以条石鲷（Oplegnathus fasciatus）的血细胞来测量DNA损伤。还对原油组分（伊朗重质原油，IHC）、风化油和六个亚组分（F2.1 - F2.6）进行了DNA损伤检测。发现新德瑞沙丘地区的DNA损伤最为严重，与原油相比，F2组分中风化油的DNA损伤降低至40%。各组分总和的DNA损伤高于沉积物的有机提取物，表明基因毒性化合物之间存在拮抗作用。本研究证实，在漏油事件发生长达5年后，受严重影响地区的沉积物中潜在基因毒性仍然存在。
