Zhou Ji, Shi Ruiyan, Liu Jianxu, Wang Rui, Xu Yufang, Qian Xuhong
East China University of Science and Technology, School of Pharmacy, Shanghai, China.
Org Biomol Chem. 2015 May 21;13(19):5344-8. doi: 10.1039/c5ob00209e.
A fluorescent probe for sensitive detection of hydrazine based on an ESIPT mechanism and a substitution-cyclization-elimination cascade was developed. After the addition of hydrazine, an approximately 50-fold enhancement in fluorescence intensity at 465 nm was observed and the subsequent decrease at 375 nm was observed in 10 min with a detection limit of 0.147 μM. We also detected hydrazine in HeLa cells successfully.
基于激发态分子内质子转移（ESIPT）机制和取代-环化-消除级联反应，开发了一种用于灵敏检测肼的荧光探针。加入肼后，在465 nm处观察到荧光强度增强约50倍，随后在10分钟内观察到375 nm处荧光强度下降，检测限为0.147 μM。我们还成功地在HeLa细胞中检测到了肼。
