Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2017 Jan 1;162:225-231. doi: 10.1016/j.talanta.2016.10.026. Epub 2016 Oct 4.
A turn-on fluorescent probe (Naphsulf-O) for hydrazine was developed by protecting the hydroxy group of the fluorophore 6-acetyl-2-hydroxynaphthalene via O-4-nitrobenzenesulfonylation, where 4-nitrobenzene was used as a fluorescence quenching moiety as well as an electrophile. Upon nucleophilic aromatic substitution (NAS) reaction of hydrazine toward the probe, the protecting group was removed and fluorophore was released. The probe exhibits a large Stokes shift, excellent selectivity and high sensitivity for hydrazine detection in aqueous solution with a detection limit of 0.716 ppb (22nM), which is of great importance in both environmental and biological system. Furthermore, it was successfully applied to imaging of hydrazine in living cells.
一种用于联氨的荧光探针（Naphsulf-O）通过将荧光团 6-乙酰-2-羟基萘的羟基通过 O-4-硝基苯磺酰化进行保护而开发，其中 4-硝基苯既作为荧光猝灭部分又作为亲电试剂。当联氨发生亲核芳香取代（NAS）反应时，保护基团被去除并且荧光团被释放。该探针在水溶液中联氨检测中表现出大的斯托克斯位移、优异的选择性和高灵敏度，检测限为 0.716 ppb（22nM），这在环境和生物系统中都非常重要。此外，它还成功地应用于活细胞中联氨的成像。
Zotero 插件