Bennett Gwendolyn, Peterson Craig L
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA.
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA.
DNA Repair (Amst). 2015 Jun;30:38-45. doi: 10.1016/j.dnarep.2015.03.006. Epub 2015 Mar 25.
The DNA damage response to double-strand breaks (DSBs) is critical for cellular viability. Recent work has shown that a host of chromatin regulators are recruited to a DSB, and that they are important for the DNA damage response. However, the functional relationships between different chromatin regulators at DSBs remain unclear. Here we describe a conserved functional interaction among the chromatin remodeling enzyme, SWI/SNF, the NuA4 and Gcn5 histone acetyltransferases, and phosphorylation of histone H2A.X (γH2AX). Specifically, we find that the NuA4 and Gcn5 enzymes are both required for the robust recruitment of SWI/SNF to a DSB, which in turn promotes the phosphorylation of H2A.X.
对双链断裂（DSB）的DNA损伤反应对细胞活力至关重要。最近的研究表明，许多染色质调节因子被招募到DSB处，并且它们对DNA损伤反应很重要。然而，DSB处不同染色质调节因子之间的功能关系仍不清楚。在这里，我们描述了染色质重塑酶SWI/SNF、NuA4和Gcn5组蛋白乙酰转移酶以及组蛋白H2A.X磷酸化（γH2AX）之间保守的功能相互作用。具体而言，我们发现NuA4和Gcn5酶对于将SWI/SNF强力招募到DSB处都是必需的，这反过来又促进了H2A.X的磷酸化。
