Afrasiabi Shima, Moniri Rezvan, Samimi Mansooreh, Mousavi Seyed Gholamabbas
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran ; Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Iran J Microbiol. 2014 Jun;6(3):194-7.
Neisseria gonorrhoeae is the second most sexually transmitted diseases agents in developing countries. Antimicrobial resistance strains have created serious health concern. The aim of this study was to determine the frequency of endocervical gonococcal infection and antimicrobial susceptibility of N. gonorrhoeae in Kashan, Iran.
In this study, 294 endocervical swabs were collected from married women referred to the obstetrics and gynecology clinics in Kashan from December 2012 to May 2013. The samples were cultured in modified Thayer Martin in 37°C with 5-10% CO2 for 72 hours. Gram staining, oxidase, catalase and carbohydrate utilization tests were used to identify the isolated species. All isolates were tested for their susceptibilities to antimicrobials using the Kirby Bauer-disk diffusion techniques.
N. gonorrhoeae was detected in 2.38% of studied cases (95% confidence interval [CI] 1.5-3.26%). All isolates were resistance to ceftriaxone, penicillin G, ciprofloxacin, cefepime, and two isolate (28.5%) showed intermediate sensitivity to tetracycline.
Continued monitoring of prevalence of N. gonorrhoeae is important for preventing the dissemination of this microorganism. The present study emphasizes the importance of surveillance of antimicrobial resistance of N. gonorrhoeae in order to manage the rate of resistant strains and to revise the treatment policies.
淋病奈瑟菌是发展中国家第二常见的性传播疾病病原体。抗菌药物耐药菌株引发了严重的健康问题。本研究的目的是确定伊朗卡尚地区宫颈内淋病奈瑟菌感染的频率及其对抗菌药物的敏感性。
在本研究中，于2012年12月至2013年5月期间，从转诊至卡尚妇产科诊所的已婚女性中采集了294份宫颈拭子样本。样本在改良的Thayer Martin培养基中于37°C、5 - 10%二氧化碳环境下培养72小时。采用革兰氏染色、氧化酶、过氧化氢酶和碳水化合物利用试验来鉴定分离出的菌种。所有分离株均使用Kirby Bauer纸片扩散法检测其对抗菌药物的敏感性。
在所研究的病例中，2.38%检测到淋病奈瑟菌（95%置信区间[CI] 1.5 - 3.26%）。所有分离株均对头孢曲松、青霉素G、环丙沙星、头孢吡肟耐药，有两株（28.5%）对四环素表现出中度敏感性。
持续监测淋病奈瑟菌的流行情况对于预防该微生物的传播很重要。本研究强调了监测淋病奈瑟菌抗菌药物耐药性的重要性，以便控制耐药菌株的比例并修订治疗策略。
