Parivar Kazem, Baharara Javad, Sheikholeslami Azar
Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Cell J. 2015 Spring;17(1):27-36. doi: 10.22074/cellj.2015.509. Epub 2015 Apr 8.
There are several factors, like environmental agents, neurotrophic factors, serotonin and some hormones such as estrogen, affecting neurogenesis and neural differentiation. Regarding to importance of proliferation and regeneration in central nervous system, and a progressive increase in neurodegenerative diseases, cell therapy is an attractive approach in neuroscience. The aim of the present study was to investigate the effects of sex steroid hormones and basic fibroblast growth factor (bFGF) on neuronal differentiation of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs).
This experimental study was established in Kharazmi Univer- sity. BM was isolated from the bones of femur and tibia of 4-6-week old Naval Medical Research Institute (NMRI) mice, and the cells were cultured. The cells were divided into following 4 groups based on the applied treatments: I. control (no treatment), II. steroid hormones (β-estradiol, progesterone and testosterone), III. bFGF and IV. combination of steroid hormones and bFGF. Immunocytochemistry and flow cytometery analyses were applied for beta III-tubulin (β-III tubulin) and microtubule-associated proteins-2 (MAP-2) in 4 days of treatment for all groups.
The cells treated with combination of bFGF and steroid hormones represented more expressions of neural markers as compared to control and to other two groups treated with either bFGF or steroid hormones.
This study showed that BM-MSCs can express specific neural markers after receiving bFGF pretreatment that was followed by sex steroid hormones treatment. More investigations are necessary to specify whether steroid hormones and bFGF can be considered for treatment of CNS diseases and disorders.
有多种因素，如环境因子、神经营养因子、血清素以及一些激素（如雌激素），会影响神经发生和神经分化。鉴于中枢神经系统中增殖和再生的重要性，以及神经退行性疾病的不断增加，细胞治疗在神经科学领域是一种有吸引力的方法。本研究的目的是探讨性类固醇激素和碱性成纤维细胞生长因子（bFGF）对小鼠骨髓间充质干细胞（BM-MSCs）神经元分化的影响。
本实验研究在哈扎尔米大学开展。从4 - 6周龄海军医学研究所（NMRI）小鼠的股骨和胫骨中分离骨髓，并培养细胞。根据所施加的处理将细胞分为以下4组：I. 对照组（不处理），II. 类固醇激素组（β-雌二醇、孕酮和睾酮），III. bFGF组，IV. 类固醇激素与bFGF联合组。在处理4天后，对所有组应用免疫细胞化学和流式细胞术分析βIII微管蛋白（β-III tubulin）和微管相关蛋白2（MAP-2）。
与对照组以及单独使用bFGF或类固醇激素处理的其他两组相比，用bFGF和类固醇激素联合处理的细胞表现出更多的神经标志物表达。
本研究表明，BM-MSCs在接受bFGF预处理后再进行性类固醇激素处理时可表达特定的神经标志物。是否可以考虑使用类固醇激素和bFGF来治疗中枢神经系统疾病和紊乱还需要更多研究。
