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采用培养的角质形成细胞和成纤维细胞联合纤维蛋白胶自体移植治疗人类肥厚性瘢痕。

Treatment of Hypertrophic Scar in Human with Autologous Transplantation of Cultured Keratinocytes and Fibroblasts along with Fibrin Glue.

作者信息

Taghiabadi Ehsan, Mohammadi Parvaneh, Aghdami Nasser, Falah Nasrin, Orouji Zahra, Nazari Abdoreza, Shafieyan Saeed

机构信息

Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

出版信息

Cell J. 2015 Spring;17(1):49-58. doi: 10.22074/cellj.2015.511. Epub 2015 Apr 8.

DOI:10.22074/cellj.2015.511
PMID:25870834
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4393671/
Abstract

OBJECTIVE

Hypertrophic scar involves excessive amounts of collagen in dermal layer and may be painful. Nowadays, we can't be sure about effectiveness of procedure for hypertrophic scar management. The application of stem cells with natural scaffold has been the best option for treatment of burn wounds and skin defect, in recent decades. Fibrin glue (FG) was among the first of the natural biomaterials applied to enhance skin deformity in burn patients. This study aimed to identify an efficient, minimally invasive and economical transplantation procedure using novel FG from human cord blood for treatment of hypertrophic scar and regulation collagen synthesis.

MATERIALS AND METHODS

In this case series study, eight patients were selected with hypertrophic scar due to full-thickness burns. Human keratinocytes and fibroblasts derived from adult skin donors were isolated and cultured. They were tested for the expression of cytokeratin 14 and vimentin using immunocytochemistry. FG was prepared from pooled cord blood. Hypertrophic scars were extensively excised then grafted by simply placing the sheet of FG containing autologous fibroblast and keratinocytes. Histological analyses were performed using Hematoxylin and eosin (H&E) and Masson's Trichrome (MT) staining of the biopsies after 8 weeks.

RESULTS

Cultured keratinocytes showed a high level of cytokeratin 14 expression and also fibroblasts showed a high level of vimentin. Histological analyses of skin biopsies after 8 weeks of transplantation revealed re-epithelialization with reduction of hypertrophic scars in 2 patients.

CONCLUSION

These results suggest may be the use of FG from cord blood, which is not more efficient than previous biological transporters and increasing hypertrophic scar relapse, but could lead to decrease pain rate.

摘要

目的

增生性瘢痕真皮层含有过量胶原蛋白，且可能伴有疼痛。目前，我们尚不能确定增生性瘢痕治疗方法的有效性。近几十年来，将干细胞与天然支架材料联合应用一直是治疗烧伤创面和皮肤缺损的最佳选择。纤维蛋白胶（FG）是最早应用于改善烧伤患者皮肤畸形的天然生物材料之一。本研究旨在确定一种高效、微创且经济的移植方法，使用源自人脐带血的新型FG治疗增生性瘢痕并调节胶原蛋白合成。

材料与方法

在本病例系列研究中，选取了8例因深度烧伤导致增生性瘢痕的患者。分离并培养来自成年皮肤供体的人角质形成细胞和成纤维细胞。采用免疫细胞化学法检测它们细胞角蛋白14和波形蛋白的表达。从汇集的脐带血中制备FG。广泛切除增生性瘢痕，然后通过简单放置含有自体成纤维细胞和角质形成细胞的FG片进行移植。8周后，对活检组织进行苏木精和伊红（H&E）染色及Masson三色（MT）染色，进行组织学分析。

结果

培养的角质形成细胞显示出高水平的细胞角蛋白14表达，成纤维细胞也显示出高水平的波形蛋白。移植8周后皮肤活检组织的组织学分析显示，2例患者出现了再上皮化，增生性瘢痕减轻。

结论

这些结果表明，使用脐带血来源的FG可能不会比以前的生物转运体更有效，且会增加增生性瘢痕复发，但可能会降低疼痛发生率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/162d92131525/Cell-J-17-49-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/d331e9303c41/Cell-J-17-49-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/4da51969d20d/Cell-J-17-49-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/0b43acf6af8c/Cell-J-17-49-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/28efbcaf3f40/Cell-J-17-49-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/6199810387cb/Cell-J-17-49-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/d2b05a12aafc/Cell-J-17-49-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/162d92131525/Cell-J-17-49-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/d331e9303c41/Cell-J-17-49-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/4da51969d20d/Cell-J-17-49-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/0b43acf6af8c/Cell-J-17-49-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/28efbcaf3f40/Cell-J-17-49-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/6199810387cb/Cell-J-17-49-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/d2b05a12aafc/Cell-J-17-49-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96a5/4393671/162d92131525/Cell-J-17-49-g07.jpg

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