Madaan Alka, Kanjilal Satyajyoti, Gupta Arun, Sastry J L N, Verma Ritu, Singh Anu T, Jaggi Manu
Indian J Exp Biol. 2015 Mar;53(3):158-63.
Chyawanprash is an ayurvedic formulation used in Indian traditional medicinal system for its beneficial effect on human health. We investigated the immunostimulatory effects of Chyawanprash (CHY) using in vitro assays evaluating the secretion of cytokines such as Tumor Necrosis Factor-alpha (TNF-α), Interleukin-1beta (IL-1β) and Macrophage Inflammatory Protein-1-alpha (MIP-1-α) from murine bone marrow derived Dendritic Cells (DC) which play pivotal role in immunostimulation. The effects of CHY on phagocytosis in murine macrophages (RAW264.7) and Natural Killer (NK) cell activity were also investigated. At non-cytotoxic concentrations (20-500 μg/ml), CHY enhanced the secretion of all the three cytokines from DC. CHY also stimulated both, macrophage (RAW264.7) as well as NK cell activity, in vitro. In conclusion, the data substantiates the immunoprotective role of CHY at cellular level mediated by immunostimulation in key immune cells viz. dendritic Cells, macrophages and NK cells.
查亚万补汁是印度传统医学体系中使用的一种阿育吠陀配方制剂，因其对人体健康有益而被使用。我们使用体外试验研究了查亚万补汁（CHY）的免疫刺激作用，该试验评估了来自小鼠骨髓来源的树突状细胞（DC）分泌的细胞因子，如肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和巨噬细胞炎性蛋白-1-α（MIP-1-α），这些细胞因子在免疫刺激中起关键作用。还研究了CHY对小鼠巨噬细胞（RAW264.7）吞噬作用和自然杀伤（NK）细胞活性的影响。在无细胞毒性浓度（20-500μg/ml）下，CHY增强了DC分泌所有三种细胞因子的能力。CHY在体外还刺激了巨噬细胞（RAW264.7）以及NK细胞的活性。总之，数据证实了CHY在关键免疫细胞即树突状细胞、巨噬细胞和NK细胞中通过免疫刺激介导的细胞水平免疫保护作用。
