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制备一种含补骨脂素的新型脱氧腺苷构件，用于简便地固相合成补骨脂素修饰的寡核苷酸，以与给定靶序列进行序列特异性交联。

Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence.

作者信息

Pieles U, Sproat B S, Neuner P, Cramer F

机构信息

EMBL, Heidelberg, FRG.

出版信息

Nucleic Acids Res. 1989 Nov 25;17(22):8967-78. doi: 10.1093/nar/17.22.8967.

DOI:10.1093/nar/17.22.8967
PMID:2587249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC335106/
Abstract

4,5',8-Trimethylpsoralen was attached to the C8-position of deoxyadenosine via a sulfur atom and a five carbon atom linker. The modified deoxyadenosine was then converted to a protected phosphoramidite and used as unusual as a building block for solid phase oligodeoxyribonucleotide synthesis. The efficiency of the photoreaction of a psoralen-modified oligonucleotide to a complementary matrix strand reached more than 90% within a 1 hour irradiation time at a wavelength of 345 nm.

摘要

4,5',8-三甲基补骨脂素通过一个硫原子和一个含五个碳原子的连接基团连接到脱氧腺苷的C8位。然后将修饰后的脱氧腺苷转化为受保护的亚磷酰胺，并用作固相寡脱氧核糖核苷酸合成中一种不同寻常的构件。在345nm波长下照射1小时内，补骨脂素修饰的寡核苷酸与互补模板链的光反应效率达到90%以上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ae2/335106/f0e76829cc20/nar00139-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ae2/335106/401964696b8b/nar00139-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ae2/335106/f0e76829cc20/nar00139-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ae2/335106/401964696b8b/nar00139-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ae2/335106/f0e76829cc20/nar00139-0075-a.jpg

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Nucleic acid-binding molecules with high affinity and base sequence specificity: intercalating agents covalently linked to oligodeoxynucleotides.具有高亲和力和碱基序列特异性的核酸结合分子：与寡脱氧核苷酸共价连接的嵌入剂
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Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light.口服甲氧沙林与长波紫外线光化学疗法治疗银屑病
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Biochemistry. 1986 Sep 9;25(18):5092-7. doi: 10.1021/bi00366a017.
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Treatment of cutaneous T-cell lymphoma by extracorporeal photochemotherapy. Preliminary results.体外光化学疗法治疗皮肤T细胞淋巴瘤。初步结果。
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