N2,N2,7-三甲基鸟苷帽衍生物的合成。
Synthesis of N2, N2, 7-trimethylguanosine cap derivatives.
作者信息
Iwase R, Sekine M, Tokumoto Y, Ohshima Y, Hata T
机构信息
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Nucleic Acids Res. 1989 Nov 25;17(22):8979-89. doi: 10.1093/nar/17.22.8979.
Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm3(2,2,7)G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid and diphosphoric acid to give N2,N2,7-trimethylguanosine-5'-diphosphate (ppm3(2,2,7)G) and 5'-triphosphate (ppm3(2,2,7)G), respectively. Similar reactions of PhSpm3(2,2,7)G with ADP and GDP gave capped dinucleoside triphosphates, m3(2,2,7)G5'pppA and m3(2,2,7)G5'pppG, respectively.
通过使用S-苯基N2,N2,7-三甲基鸟苷5'-硫代磷酸酯（PhSpm3(2,2,7)G）作为关键中间体，合成了几种在小核RNA 5'端发现的N2,N2-7-三甲基鸟苷（m3(2,2,7G)-帽）衍生物。该化合物在磷酸和焦磷酸存在下用碘活化，分别得到N2,N2,7-三甲基鸟苷-5'-二磷酸（ppm3(2,2,7)G）和5'-三磷酸（ppm3(2,2,7)G）。PhSpm3(2,2,7)G与ADP和GDP的类似反应分别得到带帽的二核苷三磷酸，即m3(2,2,7)G5'pppA和m3(2,2,7)G5'pppG。
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