Kitamura D, Kaneko H, Miyagoe Y, Ariyasu T, Watanabe T
Department of Molecular Immunology, Kyushu University, Fukuoka, Japan.
Nucleic Acids Res. 1989 Nov 25;17(22):9367-79.
A novel cDNA clone designated as HS1, which show an expression pattern limited to human hematopoietic cells, was isolated. About 2kb mRNA of the clone was accumulated in all the mature and immature lymphoid and myeloid cell lines tested, and two of three erythroblastoid cell lines, but not in any cell lines of non-hematopoietic tissues. The same mRNA was also detected in normal lymphoid and myeloid tissues and peripheral blood lymphocytes, granulocytes and macrophages, but again not in non-hematopoietic tissues. Nucleotide sequence of the HS1 predicts a protein of 486 amino acids (Mr 53,931). N-terminal half of the protein retains unique repeating motifs, each of which shows a significant homology with the helix-turn-helix DNA-binding motif of several proteins reported previously. C-terminal half of the protein retains a region conserved between non-receptor tyrosine kinases (src family), phospholipase C(PLC)-148 and the crk oncogene product. A unique feature of HS1 suggests that the protein may be involved in signal transduction and regulation of gene expression.
分离出了一个名为HS1的新型cDNA克隆，其表达模式仅限于人类造血细胞。该克隆约2kb的mRNA在所有测试的成熟和未成熟淋巴及髓系细胞系中积累，在三个成红细胞样细胞系中的两个中也有积累，但在任何非造血组织的细胞系中均未积累。在正常淋巴和髓系组织以及外周血淋巴细胞、粒细胞和巨噬细胞中也检测到了相同的mRNA，但同样未在非造血组织中检测到。HS1的核苷酸序列预测其编码一个含有486个氨基酸（分子量为53,931）的蛋白质。该蛋白质的N端一半保留了独特的重复基序，每个基序与先前报道的几种蛋白质的螺旋-转角-螺旋DNA结合基序具有显著同源性。该蛋白质的C端一半保留了非受体酪氨酸激酶（src家族）、磷脂酶C(PLC)-148和crk癌基因产物之间保守的区域。HS1的一个独特特征表明该蛋白质可能参与信号转导和基因表达调控。
