Loh Tze Ping, Metz Michael Patrick
From the Department of Laboratory Medicine, National University Hospital, Singapore;
Division of Chemical Pathology, SA Pathology, Women's and Children's Hospital, South Australia, Australia; and School of Paediatrics and Reproductive Health, University of Adelaide, South Australia, Australia.
Am J Clin Pathol. 2015 May;143(5):683-93. doi: 10.1309/AJCPB7Q3AHYLJTPK.
Derivation of between-individual biological variation (CVg) data requires repeat sampling of the same subject, which is undesirable and challenging in children. We describe an indirect sampling (data mining) approach to obtain these data in children.
Twenty-two serum biochemistry results from 6,989 children, who visited their primary care physician in Queensland, Australia, and were tested only twice within a year were included. The CVg and index of individuality of the boys and girls were estimated by year of age, according to the procedures recommended by Fraser and Harris.
The CVg was generally higher during the first year of life and declined to reach a constant level by age 4 to 6 years, except for aspartate aminotransferase, alanine aminotransferase, γ-glutamyltransferase, and phosphate. The CVg for these tended to increase after age 10 years. Most of the serum biochemistries examined in this study had indices of individuality 0.6 or less, except sodium, anion gap, bicarbonate, and chloride, which ranged from 0.6 to 1.4. The indices of individuality were very stable across all ages.
These data are comparable to those reported by the Canadian Laboratory Initiative on Pediatric Reference Intervals study and the Ricos database for adults. This study reports the CVg trends and data for boys and girls by year of age, which have not been described previously.
个体间生物学变异（CVg）数据的推导需要对同一受试者进行重复采样，这在儿童中既不可取又具有挑战性。我们描述了一种间接采样（数据挖掘）方法来获取儿童的这些数据。
纳入了6989名在澳大利亚昆士兰州看初级保健医生的儿童的22项血清生化结果，这些儿童在一年内仅接受了两次检测。根据弗雷泽和哈里斯推荐的程序，按年龄估算男孩和女孩的CVg及个体性指数。
除天冬氨酸转氨酶、丙氨酸转氨酶、γ-谷氨酰转移酶和磷酸盐外，CVg在生命的第一年通常较高，并在4至6岁时下降至稳定水平。这些指标在10岁后趋于升高。本研究中检测的大多数血清生化指标的个体性指数为0.6或更低，除了钠、阴离子间隙、碳酸氢盐和氯，其范围为0.6至1.4。个体性指数在所有年龄段都非常稳定。
这些数据与加拿大儿科参考区间实验室倡议研究和成人的里科斯数据库报告的数据相当。本研究报告了按年龄划分的男孩和女孩的CVg趋势及数据，此前尚未有过描述。
