Qi Zhihong, Zhang Lin, Chen Yu, Ma Xinming, Gao Xuehui, Du Juan, Zhang Fang, Cheng Xinqi, Cui Wei
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China.
Institute of Basic Medicine Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Clin Chim Acta. 2015 Oct 23;450:233-6. doi: 10.1016/j.cca.2015.08.026. Epub 2015 Aug 29.
We sought to identify biological variations in the following tumor markers: pepsinogen I (PGI), pepsinogen II (PGII), carbohydrate antigen 724 (CA724), neuron-specific enolase (NSE), pro-gastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), and carbohydrate antigen 199 (CA199).
Serum samples were collected from 20 healthy Chinese individuals over 5 days. Samples were then screened for the presence of the seven aforementioned tumor markers. Within-individual coefficient of variation (CVI), between-individual coefficient of variation (CVG), confidence interval (CI) of biological variations, index of individuality (II), and the reference change value (RCV) of the seven tumor markers were calculated.
Of the 7 tumor markers, index of individuality was all <1.0. ProGRP showed the lowest CVI and CVG, at 4.75% (CI: 3.96%-5.94%) and 16.42% (CI: 12.32%-24.61%), respectively. The 95% and 99% RCVs for ProGRP were 14.68 and 19.32, respectively, and were the lowest of the markers. In contrast, the CVI and CVG for CA724 were the highest, at 16.06% (CI: 13.83%-19.17%) and 96.95% (CI: 73.73%-141.59%), respectively. The 95% and 99% RCVs for CA724 were the highest, at 45.89 and 60.41, respectively.
Our findings provide additional information regarding the biological variation of tumor markers, and could be applied in a clinical setting.
我们试图确定以下肿瘤标志物的生物学变异：胃蛋白酶原I（PGI）、胃蛋白酶原II（PGII）、糖类抗原724（CA724）、神经元特异性烯醇化酶（NSE）、胃泌素释放肽前体（ProGRP）、癌胚抗原（CEA）和糖类抗原199（CA199）。
在5天内从20名健康中国个体中采集血清样本。然后对样本进行上述七种肿瘤标志物的筛查。计算七种肿瘤标志物的个体内变异系数（CVI）、个体间变异系数（CVG）、生物学变异的置信区间（CI）、个体指数（II）和参考变化值（RCV）。
在这7种肿瘤标志物中，个体指数均<1.0。ProGRP的CVI和CVG最低，分别为4.75%（CI：3.96%-5.94%）和16.42%（CI：12.32%-24.61%）。ProGRP的95%和99%RCV分别为14.68和19.32，是所有标志物中最低的。相比之下，CA724的CVI和CVG最高，分别为16.06%（CI：13.83%-19.17%）和96.95%（CI：73.73%-141.59%）。CA724的95%和99%RCV最高，分别为45.89和60.41。
我们的研究结果提供了关于肿瘤标志物生物学变异的更多信息，可应用于临床。
