Balasubramanian Sundaravadivel, Janech Michael G, Warren Graham W
Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.
Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.
Evid Based Complement Alternat Med. 2015;2015:376029. doi: 10.1155/2015/376029. Epub 2015 Mar 19.
Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N = 10 per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P < 0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P < 0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.gov NCT02108769.
瑜伽呼吸法（YB）被认为可以减轻压力和降低血压，并增强认知过程。然而，瑜伽呼吸法在分子水平上引起的变化尚未完全明确。20名健康志愿者被随机分为两组（每组N = 10）：瑜伽呼吸法组或注意力对照组（AC）。瑜伽呼吸法组进行了两次瑜伽呼吸练习，每次10分钟，单次练习共20分钟。注意力对照组阅读20分钟他们选择的文本。在基线以及5、10、15和20分钟时收集唾液。我们最初使用质谱法（MS）发现22种蛋白质存在差异表达，随后通过蛋白质印迹法验证了恶性脑肿瘤缺失-1（DMBT1）和免疫球蛋白λ-2链C区（IGLC2）。在瑜伽呼吸法组中，7名受试者的DMBT1在10分钟和15分钟时分别升高了10倍和11倍，而在时间匹配的注意力对照组中未检测到（P < 0.05）。通过双向方差分析评估，组间和时间存在显著交互作用（P < 0.001）。通过蛋白质印迹法检测，IGLC2在瑜伽呼吸法组中也显著增加。这些数据首次证明了在急性瑜伽练习后刺激和检测唾液蛋白质生物标志物的可行性。该试验已在ClinicalTrials.gov注册，注册号为NCT02108769。
