Pickering Brad S, Tyler Shaun, Smith Greg, Burton Lynn, Li Mingyi, Dallaire André, Weingartl Hana
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
PLoS One. 2015 Apr 15;10(4):e0121274. doi: 10.1371/journal.pone.0121274. eCollection 2015.
An old world fruit bat Pteropus giganteus, held in captivity and suffering from necrosis of its wing digits, failed to respond to antibiotic therapy and succumbed to the infection. Samples submitted to the National Centre for Foreign Animal Disease were tested for viral infection. Vero E6 cells exhibited minor but unique cytopathic effects on second blind passage, and full CPE by passage four. Utilizing an unbiased random amplification technique from cell culture supernatant, we identified a bacterium belonging to the Bradyrhizobiaceae. Purification of cell culture supernatant on TY media revealed a slow growing bacterial isolate. In this study using electron microscopy, 16S rRNA gene analysis and whole genome sequencing, we identify a novel bacterial species associated with the site of infection belonging to the genus Afipia. This genus of bacteria is very diverse, with only a limited number of species characterized. Afipia felis, previously described as the etiological agent to cause cat scratch disease, and Afipia septicemium, most recently shown to cause disease in humans, highlight the potential for members of this genus to form a branch of opportunistic pathogens within the Bradyrhizobiaceae. Increased utilization of next generation sequencing and genomics will aid in classifying additional members of this intriguing bacterial genera.
一只圈养的旧大陆果蝠（印度狐蝠），翅膀手指出现坏死，对抗生素治疗无反应，最终死于感染。提交给国家外来动物疾病中心的样本进行了病毒感染检测。Vero E6细胞在第二次盲传时出现轻微但独特的细胞病变效应，传代四次后出现完全细胞病变效应。利用来自细胞培养上清液的无偏随机扩增技术，我们鉴定出一种属于慢生根瘤菌科的细菌。在TY培养基上对细胞培养上清液进行纯化，得到一种生长缓慢的细菌分离株。在本研究中，通过电子显微镜、16S rRNA基因分析和全基因组测序，我们鉴定出一种与感染部位相关的新细菌物种，属于阿菲普菌属。该属细菌种类繁多，仅有少数物种得到了表征。此前被描述为引起猫抓病病原体的猫阿菲普菌，以及最近被证明可导致人类疾病的败血症阿菲普菌，凸显了该属成员在慢生根瘤菌科内形成机会性病原体分支的潜力。下一代测序和基因组学的更多应用将有助于对这个有趣细菌属的其他成员进行分类。
