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支持蓝细菌聚球藻Synechococcus leopoliensis CCAP1405/1在琼脂培养基上生长的枯草芽孢杆菌168基因。

Genes of Bacillus subtilis 168 that Support Growth of the Cyanobacterium, Synechococcus leopoliensis CCAP1405/1 on Agar Media.

作者信息

Hayashi Shohei, Itoh Kazuhito, Suyama Kousuke

机构信息

Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 690-8504, Japan.

出版信息

Microb Ecol. 2015 Oct;70(3):849-52. doi: 10.1007/s00248-015-0610-y. Epub 2015 Apr 15.

Abstract

Synechococcus leopoliensis CCAP1405/1 cannot grow on common solid media; however, the strain can grow when co-cultured with Bacillus subtilis 168. Gene-disruptant strains of B. subtilis 168 by pMUTINs at the following sites lost the ability to support the growth of S. leopoliensis CCAP1405/1 on agar media: yxeO and yufO (transporter), yxdK (histidine kinase), sdhC (succinate dehydrogenase), yvgQR (sulfite reductase), acoB (acetoin dehydrogenase), yusE (thioredoxin), yrdA (function unknown). Involvement of the assimilatory sulfate reduction pathway was the suggested reason for loss of the function.

摘要

利奥波利斯聚球藻CCAP1405/1无法在普通固体培养基上生长;然而,该菌株与枯草芽孢杆菌168共培养时能够生长。枯草芽孢杆菌168在以下位点被pMUTINs破坏基因的菌株失去了在琼脂培养基上支持利奥波利斯聚球藻CCAP1405/1生长的能力:yxeO和yufO(转运蛋白)、yxdK(组氨酸激酶)、sdhC(琥珀酸脱氢酶)、yvgQR(亚硫酸盐还原酶)、acoB(乙偶姻脱氢酶)、yusE(硫氧还蛋白)、yrdA(功能未知)。同化型硫酸盐还原途径的参与被认为是功能丧失的原因。

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