Ismail Salmah, Dadrasnia Arezoo
Department of Biohealth Science, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
PLoS One. 2015 Apr 13;10(4):e0120931. doi: 10.1371/journal.pone.0120931. eCollection 2015.
Environmental contamination by petroleum hydrocarbons, mainly crude oil waste from refineries, is becoming prevalent worldwide. This study investigates the bioremediation of water contaminated with crude oil waste. Bacillus salamalaya 139SI, a bacterium isolated from a private farm soil in the Kuala Selangor in Malaysia, was found to be a potential degrader of crude oil waste. When a microbial population of 108 CFU ml-1 was used, the 139SI strain degraded 79% and 88% of the total petroleum hydrocarbons after 42 days of incubation in mineral salt media containing 2% and 1% of crude oil waste, respectively, under optimum conditions. In the uninoculated medium containing 1% crude oil waste, 6% was degraded. Relative to the control, the degradation was significantly greater when a bacteria count of 99 × 108 CFU ml-1 was added to the treatments polluted with 1% oil. Thus, this isolated strain is useful for enhancing the biotreatment of oil in wastewater.
主要来自炼油厂原油废料的石油碳氢化合物对环境的污染在全球范围内日益普遍。本研究调查了受原油废料污染的水的生物修复情况。从马来西亚瓜拉雪兰莪的一个私人农场土壤中分离出的芽孢杆菌属萨拉马莱亚139SI，被发现是原油废料的潜在降解菌。当使用108 CFU ml-1的微生物数量时，在含有2%和1%原油废料的矿物盐培养基中，在最佳条件下培养42天后，139SI菌株分别降解了79%和88%的总石油碳氢化合物。在含有1%原油废料的未接种培养基中，6%被降解。相对于对照，当向受1%油污染的处理中添加99×108 CFU ml-1的细菌数量时，降解显著更大。因此，这种分离菌株可用于加强废水中油的生物处理。
