Lehmann Daniel, Alter Thomas, Lehmann Laura, Uherkova Simona, Seidler Tassilo, Gölz Greta
Berl Munch Tierarztl Wochenschr. 2015 Mar-Apr;128(3-4):163-8.
This study was carried out to determine the prevalence of Arcobacter spp. in food samples in Germany. In addition, the presence of putative virulence genes and the genetic diversity was tested for Arcobacter (A.) butzleri strains isolated during this study. The prevalence of Arcobacter spp. was 34% in fish meat, 26.8% in poultry meat and 2% in minced meat (beef and pork). All investigated A. butzleri isolates carried the genes cadF, ciaB, cj1349, mviN and pldA. The gene tlyA was detectable in 97.5% of the strains. Lower detection rates were observed for hecA (47.5%), hecB (45%), iroE (40%) and irgA (35%). Genotyping by ERIC-PCR demonstrated a high genetic diversity of A. butzleri strains from different foods. In conclusion, this study shows that about one third of fish meat and poultry meat samples contained Arcobacter spp. These data highlight the need to strengthen our effort to elucidate the importance ofArcobacter on veterinary public health.
本研究旨在确定德国食品样本中弓形杆菌属的流行情况。此外，还对本研究期间分离出的布氏弓形杆菌（A. butzleri）菌株的假定毒力基因的存在情况和遗传多样性进行了检测。弓形杆菌属在鱼肉中的流行率为34%，在禽肉中为26.8%，在碎肉（牛肉和猪肉）中为2%。所有被调查的布氏弓形杆菌分离株都携带cadF、ciaB、cj1349、mviN和pldA基因。97.5%的菌株中可检测到tlyA基因。hecA（47.5%）、hecB（45%）、iroE（40%）和irgA（35%）的检测率较低。通过ERIC-PCR进行基因分型表明，来自不同食品的布氏弓形杆菌菌株具有高度的遗传多样性。总之，本研究表明，约三分之一的鱼肉和禽肉样本含有弓形杆菌属。这些数据凸显了我们需要加强努力，以阐明弓形杆菌对兽医公共卫生的重要性。
