Jribi Hela, Sellami Hanen, Hassena Amal Ben, Gdoura Radhouane
1 Laboratoire de recherche Toxicologie Microbiologie Environnementale et Santé (LR17ES06), Faculté des Sciences de Sfax, Université de Sfax, Sfax 3038, Tunisia.
2 Laboratoire de Traitement et de Valorisation des Rejets Hydriques (LTVRH), Water Researches and Technologies Center (CERTE), University of Carthage, Tourist route Soliman, BP 273-8020, Nabeul 8000, Tunisia.
J Food Prot. 2017 Oct;80(10):1705-1710. doi: 10.4315/0362-028X.JFP-16-509.
Campylobacter and Arcobacter spp. are common causes of gastroenteritis in humans; these infections are commonly due to undercooked poultry. However, their virulence mechanism is still poorly understood. The aim of this study was to evaluate the presence of genotypic virulence markers in Campylobacter and Arcobacter species using PCR. The prevalence of virulence and cytolethal distending toxin (CDT) genes was estimated in 71 Campylobacteraceae isolates. PCR was used to detect the presence of virulence genes (iam, cadF, virB1, flaA, cdtA, cdtB, and cdtC) using specific primers for a total of 45 Campylobacter isolates, including 37 C. jejuni and 8 C. coli. All the Campylobacter isolates were positive for the cadF gene. The plasmid gene virB11 was not detected in any strain. The invasion associated marker was not detected in C. jejuni. Lower detection rates were observed for flaA, cdtA, cdtB, and cdtC. The presence of nine putative Arcobacter virulence genes (cadF, ciaB, cj1349, mviN, pldA, tlyA, irgA, hecA, and hecB) was checked in a set of 22 Arcobacter butzleri and 4 Arcobacter cryaerophilus isolates. The pldA and mviN genes were predominant (88.64%). Lower detection rates were observed for tlyA (84.76%), ciaB (84.61%), cadF and cj1349 (76.92%), IrgA and hecA (61.53%), and hecB (57.69%). The findings revealed that a majority of the Campylobacteraceae strains have these putative virulence genes that may lead to pathogenic effects in humans.
弯曲杆菌属和弓形杆菌属是人类肠胃炎的常见病因；这些感染通常是由于食用未煮熟的家禽所致。然而，它们的致病机制仍知之甚少。本研究的目的是使用聚合酶链反应（PCR）评估弯曲杆菌属和弓形杆菌属中基因型毒力标记的存在情况。对71株弯曲杆菌科分离株的毒力和细胞致死扩张毒素（CDT）基因的流行情况进行了评估。使用特异性引物，通过PCR检测了总共45株弯曲杆菌分离株中毒力基因（iam、cadF、virB1、flaA、cdtA、cdtB和cdtC）的存在情况，其中包括37株空肠弯曲菌和8株大肠弯曲菌。所有弯曲杆菌分离株的cadF基因均呈阳性。在任何菌株中均未检测到质粒基因virB11。在空肠弯曲菌中未检测到侵袭相关标记。flaA、cdtA、cdtB和cdtC的检测率较低。在一组22株布氏弓形杆菌和4株嗜低温弓形杆菌分离株中检测了9个假定的弓形杆菌毒力基因（cadF、ciaB、cj1349、mviN、pldA、tlyA、irgA、hecA和hecB）的存在情况。pldA和mviN基因占主导地位（88.64%）。tlyA（84.76%）、ciaB（84.61%）、cadF和cj1349（76.92%）、IrgA和hecA（61.53%）以及hecB（57.69%）的检测率较低。研究结果表明，大多数弯曲杆菌科菌株具有这些可能导致人类致病的假定毒力基因。
