Zhang Zhi-Xing, Cheng Ze-Dong, Li Chun-Ri, Ke Ai-Jun, Chen Jia-Li, Chen Yi-Guo
Zhongguo Zhen Jiu. 2014 Dec;34(12):1208-13.
To observe the effects of acupuncture at "Feishu" (BL 13) and "Lingtai" (GV 10) on distribution taxis of paclitaxel in mice with lung cancer to discuss targeted relationship between acupoints and corresponding viscera.
According to randomized digital table, 315 SPF-grade BALB/C female mice were divided into 7 groups: blank group (group A), model group (group B), medication group (group C), acupuncture at non-acupoint group (group D), acupuncture at Feishu group (group E), acupuncture at Lingtai group (group F) and acupuncture at Feishu and Lingtai group (group G), 45 mice in each one. Except the blank group, the remaining groups were treated with N-nitroso-tris-chloroethyl urea (NTCU) to establish the model of squamous-cell carcinoma. After model establishment, group A, group B and group C were not treated with acupuncture; group A and group B were treated with intraperitoneal injection of 0.9% sodium chlorvde solution by 6 mL/kg while group C was treated with intraperitoneal injection of paclitaxel by 6 mL/kg. The group D, group E, group F and group G were treated with acupuncture at non-acupoint, "Feishu" (BL 13), "Lingtai" (GV 10) and "Feishu" (BL 13) plus "Lingtai" (GV 10), respectively, then were intraperitoneally injected with paclitaxel by 6 mL/kg. The treatment was all given once a day for continuous 10 days. 15 min, 30 min, 1 h, 2 h, 8 h, 12 h and 24 h after the treatments, 6 mice in each group were randomly selected and sacrificed to collect samples of lung, liver, spleen, kidney and heart, etc. High performance liquid chromatography was applied to measure the concentration of paclitaxel in each organ (lung, liver, spleen, kidney and heart) at different time points.
(1) The content of paclitaxel in lung, kidney and heart reached the peak at 2 h, then decreased significantly in group C, group D, group E, group F and group G; the content of paclitaxel in spleen showed downtrend at each time point. The content of paclitaxel in liver reached the peak at 2 h in group C and group D; the content of paclitaxel reached the peak at 8 h in group E, group F and group G. (2) The content of paclitaxel in lung in group E and group G was higher than that in group C and group D at each time point (all P < 0.01); the content of paclitaxel in lung in group F was higher than that in group C (P < 0.01) and group D (P < 0.01) only at time point of 2 h. The content of paclitaxel in lung in group G was higher than that in group F at each time point (all P < 0.05). There was no statistical difference between group G and group E (all P > 0.05).
Acupuncture at "Feishu" (BL 13) and "Lingtai" (GV 10) could influ- ence the metabolism of paclitaxel in lung-cancer mice, leading to distribution change in each organ. As a result, it could cause targeting effects, which is more significant at "Feishu" (BL 13) and "Lingtai" (GV 10).
观察针刺“肺俞”（BL 13）、“灵台”（GV 10）对肺癌小鼠紫杉醇分布趋向的影响，探讨穴位与相应脏腑的靶向关系。
将315只SPF级BALB/C雌性小鼠按随机数字表法分为7组：空白组（A组）、模型组（B组）、药物组（C组）、非经穴针刺组（D组）、肺俞针刺组（E组）、灵台针刺组（F组）、肺俞加灵台针刺组（G组），每组45只。除空白组外，其余各组均用N-亚硝基三氯乙脲（NTCU）造模建立肺鳞状细胞癌模型。造模后，A组、B组和C组不进行针刺；A组和B组腹腔注射0.9%氯化钠溶液6 mL/kg，C组腹腔注射紫杉醇6 mL/kg。D组、E组、F组和G组分别针刺非经穴、“肺俞”（BL 13）、“灵台”（GV 10）、“肺俞”（BL 13）加“灵台”（GV 10），然后腹腔注射紫杉醇6 mL/kg。治疗均每日1次，连续10天。治疗后15 min、30 min、1 h、2 h、8 h、12 h和24 h，每组随机选取6只小鼠处死，采集肺、肝、脾、肾、心等组织样本。采用高效液相色谱法测定不同时间点各脏器（肺、肝、脾、肾、心）中紫杉醇的浓度。
（1）C组、D组、E组、F组和G组肺、肾、心中紫杉醇含量在2 h达到峰值，随后显著下降；脾中紫杉醇含量在各时间点均呈下降趋势。C组和D组肝中紫杉醇含量在2 h达到峰值；E组、F组和G组肝中紫杉醇含量在8 h达到峰值。（2）E组和G组各时间点肺中紫杉醇含量均高于C组和D组（均P < 0.01）；F组仅在2 h时肺中紫杉醇含量高于C组（P < 0.01）和D组（P < 0.01）。G组各时间点肺中紫杉醇含量均高于F组（均P < 0.05）。G组与E组比较差异无统计学意义（均P > 0.05）。
针刺“肺俞”（BL 13）、“灵台”（GV 10）可影响肺癌小鼠紫杉醇的代谢，导致各脏器分布改变，从而产生靶向效应，以“肺俞”（BL 13）、“灵台”（GV 10）最为显著。
