Lee S L, Sun C, Lin J H, Hong C Y, Wei Y H
Department of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1989 Apr;13(2):97-101.
The conventional microscopic methods for evaluating sperm motility of domestic animals are mostly inadequate due to their subjectivity and lack of precision. Recently, a trans-membrane migration method, originally developed for the examination of human sperm motility, has substantially overcome these problems. This study investigated the applicability of the method to boar sperm motility measurement. The apparatus used was simple and consisted only of syringe plungers, poriferous membranes, and modified multi-well culture plates. It measured the proportion of sperm in the semen that moved across the membrane after incubation at 37 degrees C for 3 hr. The sperm motility as measured by this method correlated well with that measured by direct microscopic examinations. The measurement was more reliable using an 8-microns instead of a 5-microns pore-size membrane. The method was found to work equally well for the sperm motility measurement of the semen with a sperm concentration between 1.5 x 10(8)/ml and 6.0 x 10(8)/ml. The results indicate that this method is a simple, objective, quantitative, and reproducible design for the measurement of boar sperm motility.
传统的评估家畜精子活力的显微镜方法大多存在主观性且缺乏精确性，因而不够完善。最近，一种最初用于检测人类精子活力的跨膜迁移方法，已在很大程度上克服了这些问题。本研究探讨了该方法在测定公猪精子活力方面的适用性。所使用的仪器很简单，仅由注射器活塞、多孔膜和改良的多孔培养板组成。它测量了精液中在37摄氏度下孵育3小时后穿过膜的精子比例。通过这种方法测得的精子活力与直接显微镜检查测得的结果相关性良好。使用孔径为8微米而非5微米的膜进行测量更为可靠。结果发现，该方法对于精子浓度在1.5×10⁸/ml至6.0×10⁸/ml之间的精液的精子活力测量同样有效。结果表明，这种方法是一种用于测定公猪精子活力的简单、客观、定量且可重复的设计。
