快速检测白色念珠菌中导致临床棘白菌素耐药的FKS1突变
Quick Detection of FKS1 Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans.
作者信息
Dudiuk Catiana, Gamarra Soledad, Jimenez-Ortigosa Cristina, Leonardelli Florencia, Macedo Daiana, Perlin David S, Garcia-Effron Guillermo
机构信息
Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT, Santa Fe, Argentina.
Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
出版信息
J Clin Microbiol. 2015 Jul;53(7):2037-41. doi: 10.1128/JCM.00398-15. Epub 2015 Apr 15.
Abstract
A rapid molecular-based assay for the detection of the Candida albicans FKS1 gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including FKS1 hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50 C. albicans strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.
摘要
设计并评估了一种基于分子的快速检测方法，用于检测白色念珠菌中导致对棘白菌素类药物耐药的FKS1基因突变。该检测方法由一组5管多重PCR组成，能够检测最常见的耐药机制，包括FKS1热点1和热点2突变。使用50株白色念珠菌的双盲样本评估了该检测方法的性能和特异性。该检测方法的灵敏度为96%，能够检测菌株集合中包含的所有纯合突变体，表明它是一种稳健、快速且省力的方法，适用于常规临床诊断实验室。
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