Begum Shameem Ara, Upadhyaya Tirtha Nath, Rahman Taibur, Pathak Debesh Chandra, Sarma Kavita, Barua Chandana Choudhury, Bora R S
Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
Department of Anatomy and Histology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India.
Indian J Pharmacol. 2015 Mar-Apr;47(2):206-11. doi: 10.4103/0253-7613.153432.
The present study investigates the effect of oral administration of chlorpyrifos (CPF) in indigenous chicken.
The birds were divided into two groups I and II. Group I served as control and group II was treated with CPF (0.36 mg/kg) orally daily up to 12 weeks. Blood samples were assayed for hemoglobin (Hb), total erythrocyte count (TEC), total leukocyte count (TLC), differential leukocyte count, and biochemical constituents like alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholinesterase (CHE), total protein and uric acid. Representative pieces of tissues from liver and kidney were collected weekly for histopathological examination.
A significant (P < 0.01) increase of Hb, TEC, TLC, and heterophil percent and decrease of lymphocyte percent was observed. Serum ALP, AST, ALT, and uric acid increased significantly and CHE values decreased significantly in CPF treated birds. The protein level remained similar. Uric acid level was found to be increased significantly in the treated group. The results indicate that chronic CPF intoxication produces hematological, biochemical, and pathological changes in treated birds.
本研究调查口服毒死蜱（CPF）对本地鸡的影响。
将鸡分为两组，即I组和II组。I组作为对照组，II组每天口服CPF（0.36毫克/千克），持续12周。对血液样本进行血红蛋白（Hb）、红细胞总数（TEC）、白细胞总数（TLC）、白细胞分类计数以及碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、胆碱酯酶（CHE）、总蛋白和尿酸等生化成分的检测。每周采集肝脏和肾脏的代表性组织样本进行组织病理学检查。
观察到Hb、TEC、TLC和嗜异性粒细胞百分比显著增加（P < 0.01），淋巴细胞百分比降低。在CPF处理的鸡中，血清ALP、AST、ALT和尿酸显著升高，CHE值显著降低。蛋白质水平保持相似。发现处理组尿酸水平显著升高。结果表明，慢性CPF中毒会使处理后的鸡出现血液学、生化和病理学变化。
