Tanvir E M, Afroz R, Chowdhury Maz, Gan S H, Karim N, Islam M N, Khalil M I
Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Bangladesh Agrochemicals and Environmental Research Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Bangladesh Department of Biochemistry, Gono Bishwabidyalay, Mirzanagar, Savar, Bangladesh.
Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Bangladesh.
Hum Exp Toxicol. 2016 Sep;35(9):991-1004. doi: 10.1177/0960327115614384. Epub 2015 Oct 30.
This study investigated the main target sites of chlorpyrifos (CPF), its effect on biochemical indices, and the pathological changes observed in rat liver and kidney function using gas chromatography/mass spectrometry. Adult female Wistar rats (n = 12) were randomly assigned into two groups (one control and one test group; n = 6 each). The test group received CPF via oral gavage for 21 days at 5 mg/kg daily. The distribution of CPF was determined in various organs (liver, brain, heart, lung, kidney, ovary, adipose tissue, and skeletal muscle), urine and stool samples using GCMS. Approximately 6.18% of CPF was distributed in the body tissues, and the highest CPF concentration (3.80%) was found in adipose tissue. CPF also accumulated in the liver (0.29%), brain (0.22%), kidney (0.10%), and ovary (0.03%). Approximately 83.60% of CPF was detected in the urine. CPF exposure resulted in a significant increase in plasma transaminases, alkaline phosphatase, and total bilirubin levels, a significant reduction in total protein levels and an altered lipid profile. Oxidative stress due to CPF administration was also evidenced by a significant increase in liver malondialdehyde levels. The detrimental effects of CPF on kidney function consisted of a significant increase in plasma urea and creatinine levels. Liver and kidney histology confirmed the observed biochemical changes. In conclusion, CPF bioaccumulates over time and exerts toxic effects on animals.
本研究使用气相色谱/质谱法，调查了毒死蜱（CPF）的主要靶位点、其对生化指标的影响以及在大鼠肝脏和肾脏功能中观察到的病理变化。成年雌性Wistar大鼠（n = 12）被随机分为两组（一组为对照组，一组为试验组；每组n = 6）。试验组大鼠每天经口灌胃给予5 mg/kg的CPF，持续21天。使用气相色谱-质谱联用仪（GCMS）测定CPF在各种器官（肝脏、大脑、心脏、肺、肾脏、卵巢、脂肪组织和骨骼肌）、尿液和粪便样本中的分布情况。约6.18%的CPF分布在身体组织中，脂肪组织中CPF浓度最高（3.80%）。CPF还在肝脏（0.29%）、大脑（0.22%）、肾脏（0.10%）和卵巢（0.03%）中蓄积。约83.60%的CPF在尿液中被检测到。CPF暴露导致血浆转氨酶、碱性磷酸酶和总胆红素水平显著升高，总蛋白水平显著降低，脂质谱发生改变。CPF给药引起的氧化应激也表现为肝脏丙二醛水平显著升高。CPF对肾功能的有害影响包括血浆尿素和肌酐水平显著升高。肝脏和肾脏组织学检查证实了观察到的生化变化。总之，CPF会随着时间生物蓄积，并对动物产生毒性作用。
