使用体内激发试验评估通过血凝抑制和乳胶凝集法测定的风疹免疫力。

Use of in vivo challenge to assess rubella immunity determined by haemagglutination inhibition and latex agglutination.

作者信息

Grangeot-Keros L, Badur S, Pons J C, Duthu S, Pillot J

机构信息

Department of Microbiology and Immunology, Hôpital Antoine Béclère, Clamart, France.

出版信息

Res Virol. 1989 Sep-Oct;140(5):437-42. doi: 10.1016/s0923-2516(89)80121-9.

DOI:10.1016/s0923-2516(89)80121-9

PMID:2587849

Abstract

In order to estimate the protection afforded by low levels of rubella antibody, pregnant women were tested by haemagglutination inhibition (HAI) and latex agglutination (LA). Fifty-nine women whose sera were HAI-negative (less than 25 IU/ml) or weakly positive (25 IU/ml) received rubella vaccine (RA 27/3) after delivery. Serum samples were collected one week and one month after immunization and tested for rubella total antibody and rubella IgM and IgA antibody. No patients found positive by HAI and LA showed any evidence of primary immunization. Furthermore, rubella IgM antibody was never detected after immunization in women who were HAI-negative and LA-positive during pregnancy. These results show that low antibody titres detected by LA are sufficient to protect individuals against infection with the attenuated virus. It is conceivable that such low titres also ensure protection against natural rubella infection.

摘要

为了评估低水平风疹抗体所提供的保护作用，对孕妇进行了血凝抑制试验（HAI）和乳胶凝集试验（LA）检测。59名血清HAI阴性（低于25 IU/ml）或弱阳性（25 IU/ml）的妇女在分娩后接种了风疹疫苗（RA 27/3）。在免疫后1周和1个月采集血清样本，检测风疹总抗体、风疹IgM和IgA抗体。HAI和LA检测呈阳性的患者均未显示初次免疫的任何证据。此外，孕期HAI阴性且LA阳性的妇女在免疫后从未检测到风疹IgM抗体。这些结果表明，LA检测到的低抗体滴度足以保护个体免受减毒病毒感染。可以想象，这样的低滴度也能确保预防自然风疹感染。