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电针在脊髓损伤修复中的作用：抑制Notch信号通路并促进神经干细胞增殖。

Electroacupuncture in the repair of spinal cord injury: inhibiting the Notch signaling pathway and promoting neural stem cell proliferation.

作者信息

Geng Xin, Sun Tao, Li Jing-Hui, Zhao Ning, Wang Yong, Yu Hua-Lin

机构信息

Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China.

出版信息

Neural Regen Res. 2015 Mar;10(3):394-403. doi: 10.4103/1673-5374.153687.

DOI:10.4103/1673-5374.153687
PMID:25878587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4396101/
Abstract

Electroacupuncture for the treatment of spinal cord injury has a good clinical curative effect, but the underlying mechanism is unclear. In our experiments, the spinal cord of adult Sprague-Dawley rats was clamped for 60 seconds. Dazhui (GV14) and Mingmen (GV4) acupoints of rats were subjected to electroacupuncture. Enzyme-linked immunosorbent assay revealed that the expression of serum inflammatory factors was apparently downregulated in rat models of spinal cord injury after electroacupuncture. Hematoxylin-eosin staining and immunohistochemistry results demonstrated that electroacupuncture contributed to the proliferation of neural stem cells in rat injured spinal cord, and suppressed their differentiation into astrocytes. Real-time quantitative PCR and western blot assays showed that electroacupuncture inhibited activation of the Notch signaling pathway induced by spinal cord injury. These findings indicate that electroacupuncture repaired the injured spinal cord by suppressing the Notch signaling pathway and promoting the proliferation of endogenous neural stem cells.

摘要

电针治疗脊髓损伤具有良好的临床疗效，但其潜在机制尚不清楚。在我们的实验中，对成年Sprague-Dawley大鼠的脊髓进行60秒的夹闭。对大鼠的大椎穴（GV14）和命门穴（GV4）进行电针治疗。酶联免疫吸附测定显示，电针治疗后脊髓损伤大鼠模型中血清炎症因子的表达明显下调。苏木精-伊红染色和免疫组织化学结果表明，电针促进了大鼠损伤脊髓中神经干细胞的增殖，并抑制了它们向星形胶质细胞的分化。实时定量PCR和蛋白质印迹分析表明，电针抑制了脊髓损伤诱导的Notch信号通路的激活。这些发现表明，电针通过抑制Notch信号通路和促进内源性神经干细胞的增殖来修复损伤的脊髓。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/80f2f20e241e/NRR-10-394-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/3f717284715f/NRR-10-394-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/0d60dc34736d/NRR-10-394-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/cb71f09cb1f8/NRR-10-394-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/42f949ba467c/NRR-10-394-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/80f2f20e241e/NRR-10-394-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/3f717284715f/NRR-10-394-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/0d60dc34736d/NRR-10-394-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/cb71f09cb1f8/NRR-10-394-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/42f949ba467c/NRR-10-394-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c6/4396101/80f2f20e241e/NRR-10-394-g006.jpg

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Syst Rev. 2013 Feb 22;2:14. doi: 10.1186/2046-4053-2-14.
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Development of a multi-electrode array for spinal cord epidural stimulation to facilitate stepping and standing after a complete spinal cord injury in adult rats.用于脊髓硬膜外刺激的多电极阵列的开发，以促进成年大鼠完全性脊髓损伤后的行走和站立。
J Neuroeng Rehabil. 2013 Jan 21;10:2. doi: 10.1186/1743-0003-10-2.
3
Notch receptor-ligand binding and activation: insights from molecular studies.
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Front Neurol. 2022 Sep 15;13:936744. doi: 10.3389/fneur.2022.936744. eCollection 2022.
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Meta-Analysis on the Effects of Electric Acupuncture on Neural Functional Recovery and Related Pathways of Rats after Spinal Cord Injury.电针对脊髓损伤后神经功能恢复及相关通路影响的Meta 分析
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