Kumar Aravind, Chandra Rampalli Viswa, Reddy Aileni Amarender, Reddy Bavigadda Harish, Reddy Chakravarthy, Naveen Anumala
Department of Periodontics, SVS Institute of Dental Sciences, Mahabubnagar, Andhra Pradesh, India.
Dent Res J (Isfahan). 2015 Mar-Apr;12(2):127-35.
The objective of this study was to evaluate the antiinflammatory, antiinfective and clinical properties of amniotic membrane (AM) when used for guided tissue regeneration (GTR) in contained interdental defects.
A total of 30 subjects participated in this study. Two sites in each subject were randomly assigned into each of the following experimental groups; test group: AM with bone graft and control group: Bone graft only. Clinical parameters included recording site-specific measures of plaque, gingivitis, probing pocket depth (PPD), and clinical attachment loss (CAL). The levels of interleukin-1β (IL-1β) and human beta-defensin-2 (hBD-2) levels in gingival crevicular fluid (GCF) from the test and control sites were measured by using commercially available enzyme linked immunosorbent assay kits. The evaluation of bone fill was performed by using digital subtraction technique and morphometric area analysis. One-way analysis of variance followed by the post-hoc test was used for intragroup and intergroup comparison. A P < 0.05 was considered as statistically significant.
Combination therapy using an AM increased bone fill and reduced PPD and CAL when compared to controls. AM also resulted in a significant reduction of GCF IL-1β levels and insignificant increase in the hBD-2 levels.
From this trial conducted over a period of 24 weeks, AM demonstrated a marked antiinflammatory effect and its use resulted in an improvement in periodontal parameters. AM has the potential to function as a barrier for GTR and the unique properties associated with this material can augment its potential as a matrix for periodontal regeneration.
本研究的目的是评估羊膜（AM）用于封闭性牙间缺损引导组织再生（GTR）时的抗炎、抗感染及临床特性。
共有30名受试者参与本研究。将每位受试者的两个部位随机分配至以下各实验组；试验组：AM联合骨移植；对照组：仅骨移植。临床参数包括记录特定部位的菌斑、牙龈炎、探诊深度（PPD）及临床附着丧失（CAL）情况。使用市售酶联免疫吸附测定试剂盒测量试验组和对照组部位龈沟液（GCF）中白细胞介素-1β（IL-1β）和人β-防御素-2（hBD-2）的水平。采用数字减法技术和形态计量面积分析对骨填充情况进行评估。组内和组间比较采用单因素方差分析及事后检验。P < 0.05被认为具有统计学意义。
与对照组相比，使用AM的联合治疗增加了骨填充，降低了PPD和CAL。AM还使GCF中IL-1β水平显著降低，hBD-2水平有不显著升高。
在为期24周的该试验中，AM显示出显著的抗炎作用，其应用使牙周参数得到改善。AM有潜力作为GTR的屏障发挥作用，且与该材料相关的独特特性可增强其作为牙周再生基质的潜力。
