Shakib Pouyan Amini, Ensani Fereshteh, Abdirad Afshin, Valizadeh Bahareh, Seyedmajidi Maryam, Sum Shima
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran ; Department of Oral and Maxillofacial Pathology, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Dent Res J (Isfahan). 2015 Mar-Apr;12(2):181-6.
Considering molecular target therapy concept in the treatment of oral squamous cell carcinoma (OSCC), many attempts have been performed to introduce an effective molecular marker during recent years. Several investigations have emphasized on the role of CD44 in various cancers and few studies have mentioned CD24 and CD74. The purpose of this study was to investigate the relationship between CD44, CD24 and CD74 expressions and several clinical or histopathological factors in OSCC patients.
In our analytical cross-sectional study, forty primary OSCC specimens were immunohistochemically stained for CD44, CD24, and CD74 proteins. Then, the relationship between their expressions and age, sex, lymph node metastasis, and histopathologic grading was statistically analyzed using Mann-Whitney nonparametric and t-test. Furthermore, P < 0.05 was considered as significant.
CD44 and CD74 proteins were significantly over-expressed in OSCC patients with high grade (P = 0.001 and P = 0.001) as compared to those with low grade. Furthermore, CD74 immunoreactivity showed significantly higher expression in patients with lower age (P = 0.039). Considering lymph node metastasis, we observed significant overexpression of CD74 in patients with no lymph node involvement (P = 0.033).
Our observations support the significant role of membranous CD44 protein in progression of OSCC and also introduce CD74 protein as a probable interfering factor in different aspects of OSCC.
考虑到分子靶向治疗概念在口腔鳞状细胞癌（OSCC）治疗中的应用，近年来人们进行了许多尝试以引入有效的分子标志物。多项研究强调了CD44在各种癌症中的作用，而提及CD24和CD74的研究较少。本研究的目的是探讨OSCC患者中CD44、CD24和CD74表达与若干临床或组织病理学因素之间的关系。
在我们的分析性横断面研究中，对40例原发性OSCC标本进行了CD44、CD24和CD74蛋白的免疫组织化学染色。然后，使用Mann-Whitney非参数检验和t检验对它们的表达与年龄、性别、淋巴结转移和组织病理学分级之间的关系进行统计学分析。此外，P < 0.05被认为具有统计学意义。
与低级别OSCC患者相比，高级别OSCC患者中CD44和CD74蛋白显著过度表达（P = 0.001和P = 0.001）。此外，CD74免疫反应性在年龄较小的患者中表达显著更高（P = 0.039）。考虑淋巴结转移情况，我们观察到无淋巴结受累患者中CD74显著过度表达（P = 0.033）。
我们的观察结果支持膜性CD44蛋白在OSCC进展中的重要作用，并将CD74蛋白作为OSCC不同方面可能的干扰因素。
