Ayub Ahmar, Poulose Ninu, Raju Raghavan
Department of Laboratory Sciences, Georgia Regents University, Augusta, Georgia, United States of America.
Department of Surgery, Georgia Regents University, Augusta, Georgia, United States of America.
Mol Med. 2015 Apr 13;21(1):305-12. doi: 10.2119/molmed.2015.00013.
Resveratrol has been shown to potentiate mitochondrial function and extend longevity; however, there is no evidence to support whether resveratrol can improve survival or prolong life following hemorrhagic shock. We sought to determine whether (a) resveratrol can improve survival following hemorrhage and resuscitation and (b) prolong life in the absence of resuscitation. Using a hemorrhagic injury (HI) model in the rat, we describe for the first time that the naturally occurring small molecule, resveratrol, may be an effective adjunct to resuscitation fluid. In a series of three sets of experiments we show that resveratrol administration during resuscitation improves survival following HI (p < 0.05), resveratrol and its synthetic mimic SRT1720 can significantly prolong life in the absence of resuscitation fluid (<30 min versus up to 4 h; p < 0.05), and resveratrol as well as SRT1720 restores left ventricular function following HI. We also found significant changes in the expression level of mitochondria-related transcription factors Ppar-α and Tfam, as well as Pgc-1α in the left ventricular tissues of rats subjected to HI and treated with resveratrol. The results indicate that resveratrol is a strong candidate adjunct to resuscitation following severe hemorrhage.
白藜芦醇已被证明可增强线粒体功能并延长寿命；然而，尚无证据支持白藜芦醇在失血性休克后能否提高生存率或延长生命。我们试图确定：（a）白藜芦醇能否在出血和复苏后提高生存率；（b）在未进行复苏的情况下能否延长生命。使用大鼠失血性损伤（HI）模型，我们首次描述了天然存在的小分子白藜芦醇可能是复苏液的有效辅助剂。在一系列三组实验中，我们表明复苏期间给予白藜芦醇可提高HI后的生存率（p<0.05），白藜芦醇及其合成类似物SRT1720在无复苏液的情况下可显著延长生命（<30分钟对长达4小时；p<0.05），并且白藜芦醇以及SRT1720可在HI后恢复左心室功能。我们还发现，在遭受HI并用白藜芦醇治疗的大鼠左心室组织中，线粒体相关转录因子Ppar-α、Tfam以及Pgc-1α的表达水平有显著变化。结果表明，白藜芦醇是严重出血后复苏的有力候选辅助剂。
